Propionyllysine

Manufactured by PTM Biolabs

Propionyllysine is a chemical compound that can be used in various laboratory applications. It is a modified lysine residue where a propionyl group is attached to the epsilon-amino group of the lysine side chain. Propionyllysine can be utilized as a research tool in biochemical and biophysical studies, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Succinyl lysine, by PTM Biolabs (1 mentions) Nupage 4 to 12 gels, by Thermo Fisher Scientific (1 mentions) Acetyl lysine, by Cell Signaling Technology (1 mentions) Odyssey scanner, by LI COR (1 mentions) β actin, by Merck Group (1 mentions) Horseradish peroxidase hrp conjugated anti rabbit igg, by Cell Signaling Technology (1 mentions) Malonyl lysine, by PTM Biolabs (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Malonyl coa, by Merck Group (1 mentions)

2 protocols using propionyllysine

Detecting Post-Translational Modifications

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Cells were harvested and lysed in TRIS‐HCL pH 7.4 with 1% triton X‐100 containing protease inhibitors and deacylase inhibitors (1 μM trichostatin A and 20 mM nicotinamide). Lysates were sonicated 5 times 2 seconds at 40 kHz amplitude on ice. Protein concentrations were determined using Pierce BCA protein assay kit (Thermofisher) and equal protein amounts were loaded on NuPAGE 4% to 12% gels (Invitrogen), transferred to nitrocellulose membrane, blocked in 3% BSA in PBS with 0.1% Tween‐20 at room temperature and incubated overnight with antibodies in the same buffer at 4°C. Primary antibodies used: β‐actin (#A5441, Sigma‐Aldrich), propionyllysine (#201, PTM biolabs), succinyllysine (#401, PTM biolabs), acetyllysine (#9441, Cell Signalling), histone 3 propionyllysine 23 (#613987, Active Motif), histone 3 acetyllysine 23 (#07‐355, Millipore). IR‐dye based secondary antibodies (LICOR) were used to detect antibody signals using Odyssey scanner (LICOR).

Lagerwaard B., Pougovkina O., Bekebrede A.F., te Brinke H., Wanders R.J., Nieuwenhuizen A.G., Keijer J, & de Boer V.C. (2020). Increased protein propionylation contributes to mitochondrial dysfunction in liver cells and fibroblasts, but not in myotubes. Journal of Inherited Metabolic Disease, 44(2), 438-449.

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Biochemical Characterization of SIRT3 and SIRT5

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Malonyl-CoA, propionyl-CoA, NAD⁺ and a protease inhibitor cocktail mixture were obtained from MilliporeSigma (St. Louis, MO). Malonyllysine (Cat# PTM-901) and propionyllysine (Cat# PTM-201) antibodies were purchased from PTM Biolabs (Chicago, IL). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Cat# AB2302) was purchased from MilliporeSigma. All other antibodies were from Cell Signaling Technology (Danvers, MA): β-actin (Cat# 4970L), histone H3 (Cat# 4499S), superoxide dismutase 2 (SOD2, Cat# 1314S), SIRT3 and 5 (Antibody Sampler Kit Cat# 9787) and horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (Cat# 7074S). Recombinant His-tagged SIRT3 (Cat# 10011194) and GST-tagged SIRT5 (Cat# 10318) were from Cayman Chemical (Ann Arbor, MI). All other chemicals used in this study were of analytical grade.

Nahomi R.B., Nandi S.K., Rakete S., Michel C., Fritz K.S, & Nagaraj R.H. (2019). Lysine malonylation and propionylation are prevalent in human lens proteins. Experimental eye research, 190, 107864.

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