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Yap taz antibodies

Manufactured by Santa Cruz Biotechnology
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The YAP/TAZ antibodies are laboratory reagents used to detect and study the expression of the YAP (Yes-Associated Protein) and TAZ (Transcriptional Coactivator with PDZ-Binding Motif) proteins in biological samples. These proteins are transcriptional regulators involved in cellular processes such as organ development, tissue homeostasis, and cancer.

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Lab products found in correlation

Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (2 mentions) Poly l ornithine, by Merck Group (2 mentions) Fv1000 confocal microscopy, by Olympus (2 mentions) Alexa fluor 488 secondary antibody, by Thermo Fisher Scientific (2 mentions) Ar 5.11.00 imaging software, by Olympus (2 mentions)

Spelling variants of this product

YAP/TAZ (8 citations)

2 protocols using yap taz antibodies

1

Quantifying YAP/TAZ Nuclear Localization

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HEK293A and A549 cells were seeded on poly-L-ornithine (Sigma)-coated coverslips to the high density in 12 well plates. After heat shock at 43°C for the indicated time, cells were successively fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS for 10 min at room temperature. Cells were blocked in PBS with 2% BSA and 2% goat serum for 30 min at room temperature and incubated overnight at 4°C in primary YAP/TAZ antibodies (Santa Cruz, 1:200) diluted in 1% BSA. After three washes with PBS, Alexa Fluor 488 secondary antibodies (Life Technologies, 1:1000) were diluted in 1% BSA and incubated for 1 h in the dark at room temperature. Slides were mounted with Prolong Gold antifade reagent with DAPI (Life Technologies). Images were captured with Olympus FV1000 confocal microscopy, immunofluorescence data were collected by NIS-Elements AR 5.11.00 imaging software and the signals from channels were merged by ImageJ (1.52a) software.
Luo M., Meng Z., Moroishi T., Lin K.C., Shen G., Mo F., Shao B., Wei X., Zhang P., Wei Y, & Guan K.L. (2020). Heat stress activates YAP/TAZ to induce the heat shock transcriptome. Nature cell biology, 22(12), 1447-1459.
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2

Quantifying YAP/TAZ Nuclear Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293A and A549 cells were seeded on poly-L-ornithine (Sigma)-coated coverslips to the high density in 12 well plates. After heat shock at 43°C for the indicated time, cells were successively fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS for 10 min at room temperature. Cells were blocked in PBS with 2% BSA and 2% goat serum for 30 min at room temperature and incubated overnight at 4°C in primary YAP/TAZ antibodies (Santa Cruz, 1:200) diluted in 1% BSA. After three washes with PBS, Alexa Fluor 488 secondary antibodies (Life Technologies, 1:1000) were diluted in 1% BSA and incubated for 1 h in the dark at room temperature. Slides were mounted with Prolong Gold antifade reagent with DAPI (Life Technologies). Images were captured with Olympus FV1000 confocal microscopy, immunofluorescence data were collected by NIS-Elements AR 5.11.00 imaging software and the signals from channels were merged by ImageJ (1.52a) software.
Luo M., Meng Z., Moroishi T., Lin K.C., Shen G., Mo F., Shao B., Wei X., Zhang P., Wei Y, & Guan K.L. (2020). Heat stress activates YAP/TAZ to induce the heat shock transcriptome. Nature cell biology, 22(12), 1447-1459.
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