Eclipse xdb c18 column

Manufactured by Phenomenex

The Eclipse XDB-C18 column is a reversed-phase high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. It features a C18 stationary phase bonded to a high-purity, high-surface-area silica substrate. The column is suitable for use with a variety of mobile phase conditions, including aqueous, organic, and mixed solvents.

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Lab products found in correlation

C18 guard column, by Phenomenex (2 mentions) 1100 series hplc system, by Agilent Technologies (1 mentions) Agilent chemstation software, by Agilent Technologies (1 mentions)

2 protocols using eclipse xdb c18 column

HPLC Analysis of Unknown Compounds

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Chromatographic analysis was done on Agilent Technologies 1100 series HPLC system, with a quaternary pump, degasser G1322A, autosamplling injector, and a diode-array detector. The separation was operated on an Eclipse XDB-C18 column (150 × 4.6 mm, particle size 5 μm) with a C18 guard column (Phenomenex, Torrance, CA) at room temperature. The data acquisition and processing were achieved using Agilent Chemstation software.

Shokry A.A., El-Shiekh R.A., Kamel G., Bakr A.F, & Ramadan A. (2022). Bioactive phenolics fraction of Hedera helix L. (Common Ivy Leaf) standardized extract ameliorates LPS-induced acute lung injury in the mouse model through the inhibition of proinflammatory cytokines and oxidative stress. Heliyon, 8(5), e09477.

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HPLC-ESI Analysis of Compounds

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HPLC was performed at 40 °C on an Agilent Eclipse XDB-C₁₈ column (150 mm × 4.6 mm, 5 μm) preceded by a Phenomenex C₁₈ guard column (4 mm × 3.0 mm). The mobile phase consisted of an aqueous ammonium acetate buffer (5 mM, pH = 5.0) and methanol. Samples were eluted isocratically by 70% methanol for 10 min at a flow rate of 0.8 mL/min. The LC effluent was introduced into ESI source using a post-column splitting ratio of 1:3 (retained/waste).

Zhang X., Wang X., Vernikovskaya D.I., Fokina V.M., Nanovskaya T.N., Hankins G.D, & Ahmed M.S. (2014). Quantitative determination of metformin, glyburide and its metabolites in plasma and urine of pregnant patients by LC-MS/MS. Biomedical chromatography : BMC, 29(4), 560-569.

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