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Anti mouse il 4 antibody

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The Anti-mouse IL-4 antibody is a laboratory reagent used to detect and quantify the levels of interleukin-4 (IL-4) in mouse samples. IL-4 is a cytokine that plays a key role in the regulation of immune responses.

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Lab products found in correlation

Af 214 14, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Anti-IL-4 (121 citations) Mouse IL-4 (36 citations) Anti-mouse IL-4 (7 citations)

2 protocols using anti mouse il 4 antibody

1

Murine IL-4 Immune Modulation

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Animal-free recombinant murine IL-4 (AF-214-14, Peprotech) was suspended at a concentration of 500 μg/ml and mixed with anti-mouse IL-4 antibody (BioXcell clone 11b11) at a molar ratio of 2:1 (weight 1:5) for 1–2 minutes at room temperature to complex IL-4 with the antibody. The complex (IL-4c) was suspended in PBS to a concentration of 25μg/ml IL-4 and 125 μg/ml of 11b11. Each mouse was injected intraperitoneally with 200μl of IL-4c (5 μg IL-4 and 25 μg 11b11) on day 0 and peritoneal exudate cells (PECs) were collected on day 2.
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
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2

Murine IL-4 Immune Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal-free recombinant murine IL-4 (AF-214-14, Peprotech) was suspended at a concentration of 500 μg/ml and mixed with anti-mouse IL-4 antibody (BioXcell clone 11b11) at a molar ratio of 2:1 (weight 1:5) for 1–2 minutes at room temperature to complex IL-4 with the antibody. The complex (IL-4c) was suspended in PBS to a concentration of 25μg/ml IL-4 and 125 μg/ml of 11b11. Each mouse was injected intraperitoneally with 200μl of IL-4c (5 μg IL-4 and 25 μg 11b11) on day 0 and peritoneal exudate cells (PECs) were collected on day 2.
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
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