Cytochrome c elisa kit

Fetal bovine serum (FBS), trypsin, and Hank's solution were purchased from Gibco (Grand Island, NY, USA). Dulbecco's Modified Eagle's Medium (DMEM), Hoechst 33342, tetramethylrhodamine ethyl ester (TMRE), calcein-AM, and Cyclosporin A (CSA) were brought from KeyGEN Biotech (Nanjing, China). Mito-Tracker Red, Mito-Tracker Green, and Mito-Sox Red were obtained from Invitrogen (Carlsbad, CA, USA). Chir99021 was purchased from Selleck Chemicals (Houston, TX, USA). Edaravone, Mdivi-1, and Mito-TEMPO were from Sigma (St Louis, MO, USA). These reagents were dissolved in dimethyl sulfoxide (DMSO) and the final DMSO concentration was <0.1% v/v. Primary antibodies and secondary antibodies for western blot analyses are listed in Supplementary Tables 1 and 2. Cytochrome c ELISA kit was purchased from Cusabio (Wuhan, China) and the intracellular calcium kit was from Beyotime Biotechnology (Nanjing, China).

NXT was purchased from Shanxi Buchang Pharmaceutical Co., Ltd. (Xi'an, China) (drug approval number Z20025001; product batch number 1411145). B27, DCFH-DA, Calcein-AM, and Trizol were obtained from Invitrogen (Eugene, USA). Cell counting kit-8 and Rhod 2-AM probe were obtained from Dojindo (Kumamoto, Japan). DAF-FM DA probe and JC-1 assay kit were from Beyotime (Shanghai, China). Cytochrome-C ELISA kit and neuronal Nitric Oxide Synthase ELISA kit were obtained from Cusabio (Wuhan, China). RIPA buffer, BCA Bradford protein assay kit, poly-D-lysine, and trypsin were purchased from Solarbio (Beijing, China). TaqMan reverse transcription reagents were obtained from Applied Biosystems (Foster City, USA). Cleaved caspase-3 and p-Akt antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Bcl-2, Bax, β-actin, and HRP-conjugated secondary antibodies were purchased from Abcam (Cambridge, MA, USA).