Complete mtesr plus medium

Manufactured by STEMCELL

Complete mTeSR™ Plus medium is a basal medium used for the maintenance and expansion of human pluripotent stem cells in vitro. It is a chemically defined, serum-free, and feeder-free medium that supports the undifferentiated growth of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs).

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Lab products found in correlation

Tryple express enzyme, by Thermo Fisher Scientific (1 mentions) Dispase, by STEMCELL (1 mentions) Mtesr1, by STEMCELL (1 mentions) Ldev free hesc qualified matrigel, by Corning (1 mentions) Relesr, by STEMCELL (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Matrigel coated plate, by Corning (1 mentions) Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)

Close spelling variants of this product

MTeSR medium (173 citations) MTeSR Plus medium (106 citations) MTeSR Plus (83 citations) Complete mTeSR medium (5 citations) Complete mTeSR Plus (2 citations) MTeSR Complete (2 citations)

3 protocols using complete mtesr plus medium

Generation of hiPSC-derived Neural Stem Cells

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The human induced pluripotent stem cell (hiPSC) line bASC3 was previously generated in our laboratory from human breast adipose stem cells (Mollica et al., 2018a (link)). hiPSCs were adapted to feeder-free conditions, and cells were seeded on GTX-coated plates. hiPSC cultures were maintained following the Maintenance of Human Pluripotent Stem Cells in mTeSR™1 technical manual (STEMCELL Technologies; Cambridge, MA). Cells were maintained in complete mTeSR™ Plus medium (STEM CELL Technologies), replenished daily. Enzymatic passage was performed using Dispase (STEMCELL Technologies) according to the manufacturer’s suggested protocol. Pluripotency of the hiPSC colonies was confirmed using a Tra-1-60 live staining kit (Thermo Fisher).
Human neural stem cells (hNSCs) were generated from the is-bASC3 line using the STEMdiff™ Neural System Embryoid Body (EB) protocol (STEMCELL Technologies) following the manufacturer’s instructions. Newly formed NSCs were cultured on GTX^® coated plates and in complete StemPro^® NSC Serum Free Medium (Thermo Fisher Scientific), replenished every other day. Cells were passaged using TrypLE™ Express Enzyme (Thermo Fisher) according to the manufacturer’s suggested protocol. The identity of the newly formed hNSCs was confirmed via immunocytochemistry using the neural stem cell markers SOX2 and NESTIN.

Zamponi M., Mollica P.A., Khodour Y., Bjerring J.S., Bruno R.D, & Sachs P.C. (2023). Combined 3D bioprinting and tissue-specific ECM system reveals the influence of brain matrix on stem cell differentiation. Frontiers in Cell and Developmental Biology, 11, 1258993.

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Reprogramming A-T Fibroblasts to iPSCs

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Fibroblasts from an individual with A-T (2 years old, female) were reprogrammed into iPSCs using non-integrative Sendai virus. The patient had the following compound-heterozygous variants in ATM: hg38 chr11:g.108332838C>T; chr11:g.108347266_108353792del27. Control iPSCs from Personal Genome Project participant 1 (PGP1) were used as the control line. Both iPSC lines were mycoplasma negative, karyotypically normal, and expressed pluripotency markers OCT4, SOX2, NANOG, SSEA4, TRA-1-60. iPSCs were maintained in 6-well or 10 cm plates (Corning) coated with LDEV-free hESC-qualified Matrigel (Corning Cat# 354277) in feeder-free conditions with complete mTeSR-plus medium (STEMCELL Technologies Cat# 100–1130) in a humidified incubator (5% CO2, 37°C). iPSCs were fed fresh media daily and passaged every 3–4 days.

Lai J., Demirbas D., Kim J., Jeffries A.M., Tolles A., Park J., Chittenden T.W., Buckley P.G., Yu T.W., Lodato M.A, & Lee E.A. (2023). ATM-deficiency-induced microglial activation promotes neurodegeneration in ataxia-telangiectasia. Cell reports, 43(1), 113622.

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Culturing SH-SY5Y Neuroblastoma and iPSCs

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Human SH-SY5Y neuroblastoma cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Lonza) supplemented with 10% (v/v) foetal bovine serum (FBS) (Thermo-Fisher Scientific), 50 units/mL of penicillin and 50 μg/mL of streptomycin. Cell lines were maintained at 5% CO2 in a 37°C incubator and split every 3–4 days.
Human induced pluripotent stem cells iPSCs were maintained in Matrigel-coated plates (Corning) according to the manufacturer’s recommendations in complete mTeSR-Plus Medium (StemCell Technologies). The culture medium was replaced daily and confirmed mycoplasma free. Cells were passaged every four to six days as clumps using ReLeSR, an enzyme-free reagent for dissociation (StemCell Technologies) according to the manufacturer’s recommendations. For all the experiments in this study, iPSCs were between passage 20 and 32.

Zhang S., Cooper-Knock J., Weimer A.K., Shi M., Moll T., Marshall J.N., Harvey C., Nezhad H.G., Franklin J., dos Santos Souza C., Ning K., Wang C., Li J., Dilliott A.A., Farhan S., Elhaik E., Pasniceanu I., Livesey M.R., Eitan C., Hornstein E., Kenna K.P., Veldink J., Ferraiuolo L., Shaw P.J, & Snyder M.P. (2022). Genome-wide Identification of the Genetic Basis of Amyotrophic Lateral Sclerosis. Neuron, 110(6), 992-1008.e11.

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