In addition to routine determination of CD90+ cell content (see the “In-process controls and release tests ” section), the batches intended for use at study sites in the Czech Republic in the multinational clinical trial on peripheral artery disease (NCT03339973, Table 1 ) were additionally tested for expression of CD73, CD105, und CD44 [5 (link), 15 (link)], as required by the Czech national drug authority (State Institute for Drug Control, SÚKL). To this end, cell suspension samples were incubated with a fluorescein isothiocyanate (FITC)-conjugated anti-human CD73 antibody, an Alexa Fluor® 647-conjugated anti-human CD105 antibody, and a FITC-conjugated anti-human CD44 antibody (all from BioLegend), respectively. Fluorescence was measured by flow cytometry (BD Accuri™ C6) and percentages of CD73+ CD105+ and CD44+ cells were calculated. Pre-specified acceptance criteria were CD73, ≥ 95% [5 (link)]; CD105, determined and declared; and CD44, determined and declared.
Fitc conjugated anti human cd73 antibody
Manufactured by BioLegend
Sourced in United States
The (FITC)-conjugated anti-human CD73 antibody is a laboratory reagent used to detect and analyze the presence of CD73 on the surface of human cells. CD73 is an ectonucleotidase enzyme involved in the hydrolysis of extracellular adenosine monophosphate (AMP) to adenosine. The FITC (Fluorescein Isothiocyanate) conjugation allows for the fluorescent labeling of the antibody, enabling its detection and quantification through flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
2 protocols using fitc conjugated anti human cd73 antibody
1
Extensive Cell Surface Marker Profiling
2
Characterization of hPDLSCs Markers
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MSCs surface marker expression on hPDLSCs was evaluated using flow cytometry analysis (n = 4). The antibodies were FITC-conjugated anti-human CD105 antibody (Bio Legend, USA), FITC-conjugated anti-human CD73 antibody (Bio Legend, USA), FITC-conjugated anti-human CD90 antibody (Bio Legend, USA), FITC-conjugated anti-human CD44 antibody (Bio Legend, USA), and FITC-conjugated anti-human CD45 antibody (Bio Legend, USA). FITC-conjugated mouse IgG1κ Isotype was employed as the isotype control. The assay was performed by utilizing FACS caliber flow cytometer (BD Biosciences, USA).
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