Proteins extracted from the ankle joint synovial tissues or cell were separated by using 10% dodecyl sulfate, sodium salt-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to PVDF membrane. After blocking with 5% nonfat dry milk for 1 hour, the specific primary antibodies including βArr2 (Abcam, ab54790), NLRP3 (Abcam, ab263899), ASC (Cell Signaling Techbology (CST), #67824) and caspase-1 p20 (Adipogen, AG-20B-0042B), Phospho-NF-κB-p65 (P-p65, Abcam, ab239882), p-IκBα (CST, #4814), and IκBα (CST, #2859) were incubated on the membrane overnight at 4°C. In the next day, the membrane was added to the corresponding horseradish peroxidase-labeled secondary antibodies. Finally, the target protein was visualized by detecting the enhanced chemiluminiscent (ECL) fluorescence. GAPDH (Abcam, ab8245) was applied as the internal control.
Ab54790
Manufactured by Abcam
Sourced in United States
Ab54790 is a lab equipment product offered by Abcam. It serves as a general-purpose apparatus for laboratory applications. The core function of this product is to facilitate various experimental procedures and analyses conducted in a research or diagnostic setting.
Automatically generated - may contain errors
Lab products found in correlation
Ab8245, by Abcam (1 mentions)
Axiovert 100m, by Zeiss (1 mentions)
Lsm 510, by Zeiss (1 mentions)
Nbp1 00858, by Novus Biologicals (1 mentions)
C apochromat 40 1.2 w, by Zeiss (1 mentions)
Rhodamine redtm x conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Alexa fluor 488 conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
2 protocols using ab54790
1
Analyzing Synovial Tissue Protein Expression
2
Immunofluorescence Labeling of FFA4 and β-Arrestin 2
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Cells were labeled with antibodies against FFA4 (NBP1-00858, 1:500, Novus Biologicals, LLC, CO, USA) and β-arrestin 2 (ab54790, 1:500, Abcam, Cambridge, MA, USA). The secondary antibody used was Alexa Fluor 488-conjugated anti-rabbit IgG (1:1000, Jackson ImmunoResearch, West Grove, PA, USA) and Rhodamine RedTM-X-conjugated anti-mouse IgG (1:1000, Jackson ImmunoResearch, West Grove, PA, USA). A confocal scanning module (LSM510, Carl Zeiss, Germany) mounted on a fluorescence microscope (AXIOVERT 100 M, Carl Zeiss, Germany) and a C-Apochromat 40×/1.2 W (Carl Zeiss, Germany) was used34 (link).
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