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Ag25b0006c100

Manufactured by Thermo Fisher Scientific
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The AG25B0006C100 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform core laboratory functions. Detailed specifications and intended use are not available.

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Lab products found in correlation

Alexa fluor 594 conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) A1r ti2 confocal system, by Nikon (1 mentions) Cytotox 96 non radioactive cytotoxicity assay kit, by Promega (1 mentions) Tnf α, by BioLegend (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by BioLegend (1 mentions)

2 protocols using ag25b0006c100

1

ASC Oligomerization and Speck Assay

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ASC speck staining and ASC oligomerization assay were carried out as described (Chen et al., 2019). The primary anti‐ASC antibody (Adipogen, San Diego, CA, USA, AG25B0006C100, 1:200) and Alexa‐Fluor‐594‐conjugated secondary antibody (Invitrogen, A‐11037, 1:200) were used. Cell images were obtained using an A1R‐Ti2 confocal system (Nikon).
Chen X., Liu B., Li X., An T.T., Zhou Y., Li G., Wu‐Smart J., Alvarez S., Naldrett M.J., Eudy J., Kubik G., Wilson R.A., Kachman S.D., Cui J, & Yu J. (2021). Identification of anti‐inflammatory vesicle‐like nanoparticles in honey. Journal of Extracellular Vesicles, 10(4), e12069.
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2

Cytokine Profiling and Cell Death Assays

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All these assays were carried out as described [20 (link)]. The ELISA kits used included tumor necrosis factor α (TNFα) (BioLegend, San Diego, CA, USA, 430901); IL-6 (BioLegend, 431301); IL-18 (MBL, Worburn, MA, USA, D042-3); and IL-1β (eBioscience, San Diego, CA, USA, 88701388). CytoTox 96 Nonradioactive Cytotoxicity Assay kit (Promega, Madison, WI, USA) was used to assess LDH released in the medium. The primary anti-ASC antibody (Adipogen, AG25B0006C100, 1:200) and Alexa-Fluor-594-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA, A-11037, 1:200) were used for ASC speck staining.
Liu B., Lu Y., Chen X., Muthuraj P.G., Li X., Pattabiraman M., Zempleni J., Kachman S.D., Natarajan S.K, & Yu J. (2020). Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice. Nutrients, 12(2), 477.
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