Calcium chloride

Chemicals including 100% acetic acid, 4-aminoantipyrine, Coomassie Blue G-250, ethanol, ferric chloride, hydrogen chloride, meat extract, meat peptone, n-butanol, 85% phosphoric acid, potassium dihydrogen phosphate, sodium chloride, sodium dihydrogen phosphate monohydrate, and sodium hydrogen phosphate were from Merck, Darmstadt, Germany. Acetonitrile, agar, amylose-remazol brilliant blue R, bovine serum albumin, casein, 3,5-dinitrosalicylic acid, 2,2-diphenyl-1-picrylhydrazyl, Folin-Ciocalteu’s phenol-reagent, gallic acid, hydrochloric acid, hydrogen peroxide, 99% hydroxylamine hydrochloride, L-glutamic acid γ-monohydroxamate, L- Glutathione reduced, maltose, methanol, yeast extract, peptone from soybean, phenol, p-nitrophenyl butyrate, potassium sodium tartrate tetrahydrate, sodium acetate, sodium carbonate, starch, trichloroacetic acid, and Trizma Base were obtained from Sigma-Aldrich, Taufkirchen, Germany. CBZ-Glutaminylglycine was purchased from Zedira GmbH, Darmstadt, Germany. Potato dextrose agar and Mueller-Hinton broth were purchased from Biolife, Milano, Italy. Calcium chloride, D-(+)-glucose anhydrous, and iron (II) sulfate heptahydrate were obtained at Kemika, Zagreb, Croatia, while malt extract, potato dextrose broth, Triton X-100, tryptic soy broth, and tryptone were purchased from Fluka, Buchs, Switzerland.

Acetonitrile of HPLC grade was purchased from J. T. Baker (Deventer, The Netherlands). Formic acid, glacial acetic acid, and 85 % orthophosphoric acid were obtained from Fluka (Buchs, Switzerland). Acetone, calcium chloride, boric acid and 1 M sodium hydroxide solution were provided from Kemika (Zagreb, Croatia).
The standards used for the identification and quantification purposes were as follows: epigallocatechin, procyanidin B1 and procyanidin B2 (Extrasynthese, Genay Cedex, France); gallic acid, (−)-epicatechin, (+)-catechin and epicatechin-gallate (Sigma-Aldrich, St. Louis, MO, USA).
Lallzyme HC and Lallzyme EX-V isolated from Aspergillus niger were obtained from Lallemand Inc. (Montreal, Canada). The features of these enzyme preparations are given in Table 1.Table 1

Commercial names and main activities of enzyme preparations

Enzyme preparation	Main activities
	Polygalacturonase (PG U g−1)	Pectin liase (PL Ug−1)	Pectin methylestherase (PME U g−1)	Cellulase and hemicellulase
Lallzyme EX-V	4000	120	1000	+++
Lallzyme HC	3500	100	800	−