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Anti akap95

Manufactured by Santa Cruz Biotechnology

Anti-AKAP95 is a laboratory product that targets the AKAP95 protein. AKAP95 is a member of the A-kinase anchoring protein (AKAP) family, which plays a role in the regulation of cellular processes. This product can be used for research purposes to investigate the function and localization of AKAP95 in various biological systems.

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4 protocols using anti akap95

1

Immunoblotting for AKAP95 and Transcription Factors

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Rabbit polyclonal anti-AKAP95, Santa Cruz Biotechnology Cat#sc-10766; Rabbit polyclonal anti-AKAP95, Bethyl Laboratories Cat#A301–062A, Mouse monoclonal anti-GAPDH, EMD Millipore Cat#MAB374; AlexaFluor 555 conjugated goat anti-rabbit IgG, Thermo Fisher Scientific Cat#A-21428; Mouse anti-SRSF2 antibody, Abcam Cat#Ab11826; mouse anti-Pol II, 8WG16 clone, COVANCE Cat# MPY-127R; Mouse anti-pol II CTD-S2P, H5 clone, COVANCE Cat#MPYT-127R; Rabbit polyclonal anti-cyclin A, Santa Cruz Biotechnology Cat#sc-751; Rabbit polyclonal anti-DDX5, Santa Cruz Biotechnology Cat#sc-32858; Mouse monoclonal anti-hnRNP M, Santa Cruz Biotechnology Cat#sc-20002; AlexaFluor 555 conjugated goat anti-mouse IgG, Thermo Fisher Scientific Cat# A-21422. A dilution of 1:1000 was used for immunoblotting except 1:2000 for GAPDH.
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2

Antibody Validation for Protein Interactions

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Antibodies are as follows: anti-HA (Abcam, ab9110), anti-AKAP95 (Santa Cruz Biotechnology, sc-10766), anti-His (Santa Cruz Biotechnology, sc-803), anti-GAPDH (Chemicon, MAB374), anti-FLAG (Sigma, A8592, for blotting), anti-FLAG [M2 beads] (Sigma, A2220), and anti-H3K4me3 (Millipore, 07–473), anti-RBBP5 (Bethyl Laboratories, A300–109A), anti-ASH2L (Bethyl Laboratories, A300–107A), anti-WDR5 (Bethyl Laboratories, A302–430A).
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3

Antibody Sourcing and Characterization

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Antibodies were obtained commercially. From Santa Cruz Biotechnology: anti-AKAP95 (sc-10766, 1:500); anti-DDX5 (sc-32858, 1:500); anti-hnRNP M (sc-20002, 1:500); hnRNP F (sc-10045, 1:500); anti-His (sc-803, 1:500); and anti-RNA Pol II (sc-899, 1:500). From others: Anti-hnRNP A1 (Novus Biologicals, NB100–672, 1:2,000), anti-Pol II (Covance, MPY-127R for clones 8WG16, H14 and H5, 1:500), anti-hnRNP H (Bethyl Laboratories, A300–511A, 1:2,000); DDX17 (Bethyl Laboratories, A300–509A, 1:2,000); anti-HA (Abcam, ab9110, 1:1,000); anti-GAPDH (Chemicon, MAB374, 1:5,000); anti-FLAG (Sigma, A8592, 1:1,000); anti-FLAG (Sigma, A2220, M2 beads); and anti-MBP (New England BioLabs, E8032S, 1:5,000).
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4

Immunoblotting for AKAP95 and Transcription Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit polyclonal anti-AKAP95, Santa Cruz Biotechnology Cat#sc-10766; Rabbit polyclonal anti-AKAP95, Bethyl Laboratories Cat#A301–062A, Mouse monoclonal anti-GAPDH, EMD Millipore Cat#MAB374; AlexaFluor 555 conjugated goat anti-rabbit IgG, Thermo Fisher Scientific Cat#A-21428; Mouse anti-SRSF2 antibody, Abcam Cat#Ab11826; mouse anti-Pol II, 8WG16 clone, COVANCE Cat# MPY-127R; Mouse anti-pol II CTD-S2P, H5 clone, COVANCE Cat#MPYT-127R; Rabbit polyclonal anti-cyclin A, Santa Cruz Biotechnology Cat#sc-751; Rabbit polyclonal anti-DDX5, Santa Cruz Biotechnology Cat#sc-32858; Mouse monoclonal anti-hnRNP M, Santa Cruz Biotechnology Cat#sc-20002; AlexaFluor 555 conjugated goat anti-mouse IgG, Thermo Fisher Scientific Cat# A-21422. A dilution of 1:1000 was used for immunoblotting except 1:2000 for GAPDH.
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