Dulbecco s modified eagle medium dmem low glucose

Mouse BMSCs were isolated from 8-weeks-old male C57BL/6 J mice as described previously described [18 (link)]. Bone marrow was flushed out from mouse tibia and femur into Eppendorf tubes, centrifuged for 1 min at 400 g to collect the marrow cells. Cell were then filtrated through a 70-μm nylon mesh filter and cultured in 175 cm2 flasks in basal culture media (BCM) consists of RPMI-1640 medium supplemented with 12% FBS (Gibco Invitrogen, USA), 12 μM L-glutamine (Invitrogen) and 1% penicillin/streptomycin (P/S) (Gibco Invitrogen, USA). After 24 h, non-adherent cells were removed from the cultured medium, washed with PBS, and cultured in 30 ml of fresh medium in 60 cm2. Medium was changed every 3–4 days and cells were washed, trypsinized and regularly sub-cultured.
Human BMSC cells (hBMSCs) were purchased from Cell Applications Inc. (San Diego, CA). Cells were cultured according to the manufacturer’s instruction, in Dulbecco’s modified Eagle medium (DMEM)/low glucose (Sigma-Aldrich) containing 10% FBS Gibco Invitrogen, USA) and 1% penicillin/streptomycin. Non-adherent cells were removed after 2 days in culture and medium was changed every 2–3 days.

Diphenyleneiodonium (DPI), 2′,7′-dichlorofluorescin diacetate (DCFA), lipopolysaccharide (LPS), sodium nitrite solution, Griess reagent, Dulbecco’s Modified Eagle Medium (DMEM)/low glucose, phenol red-free DMEM/low glucose, penicillin/streptomycin mixture, DMSO, and fetal bovine serum (FBS) were purchased from Sigma (St. Louis, MO, USA). Glucose was purchased from Acros Organics (Fair Lawn, NJ, USA), and sodium bicarbonate was purchased from Mallinckrodt Chemicals (Phillipsburg, NJ, USA). Caco-2 cells and RAW 264.7 macrophages (cell line TIB-71™) were acquired from the American Type Culture Collection (ATCC) (Manassas, VA, USA).