Truseq nano sample

Manufactured by Illumina

The TruSeq Nano sample preparation kit is a library preparation solution designed for Illumina sequencing platforms. It provides a streamlined workflow for generating high-quality DNA libraries from a variety of input materials, including genomic DNA and FFPE samples. The kit utilizes a proprietary library preparation method to generate fragments of a specified size range, enabling efficient sequencing on Illumina instruments.

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Lab products found in correlation

Bioruptor pico sonicator, by Diagenode (3 mentions)

3 protocols using truseq nano sample

Yeast Genomic DNA Replication Timing

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Yeast genomic DNA was extracted using the smash and grab method (https://fangman-brewer.genetics.washington.edu/smash-n-grab.html). DNA was sonicated using the Bioruptor Pico sonicator (Diagenode), and the libraries were prepared according to the TruSeq Nano sample preparation guide from Illumina. To generate replication timing profiles, the ratio of uniquely mapped reads in the replicating samples to the non-replicating samples was calculated following Batrakou et al., 2020 (link). Replication profiles were generated using ggplot and smoothed using a moving average in R. The values of Trep were taken from OriDB (Siow et al., 2012 (link)).

Johnson M.C., Can G., Santos M.M., Alexander D, & Zegerman P. (2021). Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase. eLife, 10, e63589.

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Yeast Genomic DNA Extraction and Sequencing

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Santos M.M., Johnson M.C., Fiedler L, & Zegerman P. (2022). Global early replication disrupts gene expression and chromatin conformation in a single cell cycle. Genome Biology, 23, 217.

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Yeast DNA Replication Timing Profiling

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Yeast genomic DNA was extracted using the smash & grab method (https://fangman-brewer.genetics.washington.edu/smash-n-grab.html). DNA was sonicated using the Bioruptor Pico sonicator (Diagenode) and the libraries were prepared according to the TruSeq Nano sample preparation guide from Illumina. To generate replication timing profiles, the ratio of uniquely mapped reads in the replicating samples to the non-replicating samples was calculated following (Batrakou et al., 2020) . Replication profiles were generated using custom R scripts and smoothed using a moving average. The values of Trep were taken from OriDB (Siow et al., 2012) .

Johnson M.C., Can G., Santos M., Alexander D., & Zegerman P. (2020). Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase.

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