Flow cytometry was carried out by suspending MNCs in the stain buffer and treated first with FcR blocking reagent (1:100, Miltenyi Biotech) followed by incubation with the following fluorescent-conjugated antibodies (Biolegend), allophycocyanin (APC) anti-human lineage cocktail (1:500, Biolegend), phycoerythrin (PE) anti-human CD45 (1:500) and Fluorescein isothiocyanate (FITC) anti-human CD34 antibodies (1 in 250) or isotype control antibodies (1 in 500) (Biolegend, San Diego, CA, U.S.A.) for 45 min at 4°C. Dead cells were excluded using 7-AAD viability staining solution. Flow cytometry was carried out by using C6 Accuri cytometer (BD).
Fluorescein isothiocyanate fitc anti human cd34 antibodies
Manufactured by BioLegend
Sourced in United States
Fluorescein isothiocyanate (FITC) anti-human CD34 antibodies are a type of fluorescent-labeled antibody that specifically binds to the CD34 antigen expressed on the surface of human cells, such as hematopoietic stem and progenitor cells. These antibodies can be used in flow cytometry and other immunoassays to identify and quantify CD34-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Fcr blocking reagent, by Miltenyi Biotec (2 mentions)
Allophycocyanin apc anti human lineage cocktail, by BioLegend (2 mentions)
Accuri c6 cytometer, by BD (2 mentions)
Fluorescent conjugated antibodies, by BioLegend (2 mentions)
Anti human cd45, by BioLegend (1 mentions)
Isotype control antibody, by BioLegend (1 mentions)
2 protocols using fluorescein isothiocyanate fitc anti human cd34 antibodies
1
Quantifying Hematopoietic Stem Cells
2
Flow Cytometry Characterization of MNCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flow cytometry was carried out by suspending MNCs in the stain buffer and treated first with FcR blocking reagent (1:100, Miltenyi Biotec) followed by incubation with the following fluorescent-conjugated antibodies (Biolegend), allophycocyanin (APC) anti-human lineage cocktail (1:500, Biolegend), phycoerythrin (PE) anti-human CD45 (1:500) and fluorescein isothiocyanate (FITC) anti-human CD34 antibodies (1 in 250), or isotype control antibodies (1 in 500) (Biolegend, SanDiego, CA, USA) for 45 min at 4 °C. Dead cells were excluded using the 7-AAD viability staining solution. Flow cytometry was carried out by using the C6 Accuri cytometer (BD).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!