Renal function was evaluated via assessing serum levels of creatinine (Cr; ab700460), uric acid (ab65344), blood urea nitrogen (BUN; ab83362), lactate dehydrogenase (LDH; ab102526), and kidney injury molecule-1 (Kim-1; ab119597) using the specified colorimetric or ELISA kit obtained from Abcam Inc. (Waltham, MA, USA), agreeing with the manufacturer’s practices consuming a spectrophotometer (LEICA UNISTAT®; Leica Inc., Allendale, NJ, USA).
Ab119597
Manufactured by Abcam
Sourced in United States
Ab119597 is a recombinant polyclonal antibody. It is produced in vitro by recombinant technology.
Automatically generated - may contain errors
Lab products found in correlation
Dlcn20, by Abcam (2 mentions)
Ab65344, by Abcam (1 mentions)
Ab83362, by Abcam (1 mentions)
Unistat, by Leica (1 mentions)
Ab102526, by Abcam (1 mentions)
Ab119602, by Abcam (1 mentions)
Mak006 1kt, by Merck Group (1 mentions)
Mak080 1kt, by Merck Group (1 mentions)
Multiscan spectrum spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Quantikine elisa kit, by R&D Systems (1 mentions)
4 protocols using ab119597
1
Renal Function Evaluation via Biomarkers
2
Kidney Injury Biomarker Evaluation
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There were four groups in the experiment: a control group, an AngII group, a CMS group, and a RES group. The cells were cultured as earlier described, and KIM-1 and neutrophil gelatinase-associated lipocalin (NGAL) abundance in the supernatant was determined by ELISA kits obtained from R&D Systems (DLCN20, Minneapolis, MN, United States) and Abcam (ab119597). All of the operational procedures were performed in accordance with the manufacturers’ instruction.
3
Serum Biomarkers for Kidney Injury
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Blood biochemical analysis was performed according to previous investigations. The blood samples were extracted 24 h following sham or CLP operation and centrifuged at 1400 x g to prepare serum at 4°C for 10 min. SCr levels were evaluated by a creatinine enzymatic assay kit (MAK080-1KT, Sigma-Aldrich, Merck KGaA) and measured at 570 nm. BUN was detected with an enzymatic assay kit (MAK006-1KT, Sigma-Aldrich, Merck KGaA) and measured at 570 nm. The expression levels of NGAL (ab119602, Abcam) and KIM-1(ab119597, Abcam) were measured using corresponding kits and measured at 450 nm. All measurements were conducted with a multiscan spectrum spectrophotometer (Thermo Fisher Scientific Inc.)
4
Quantification of NGAL and KIM-1 in Urine
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Levels of neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule (KIM)-1 in rat urine and cell culture supernatants were measured with Quantikine ELISA kits purchased from R&D Systems (DLCN20; Minneapolis, MN, USA) and Abcam (ab119597; Cambridge, MA, USA).
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