SARS-CoV-2 spike-pseudotyped lentiviral particles, VSV-G-pseudotyped particles and human ACE2-expressing HEK293 cells were purchased from GeneCopoeia, Rockville, MD. Recombinant COVID-19 Spike protein S1 was purchased from Abeomics, San Diego, CA. While anti-SARS-CoV-2 Spike S1 antibody was bought from BioVision (Milpitas, CA), anti-human ACE2 antibody was purchased from R&D Systems (Minneapolis, MN). Anti-human MyD88 antibody was bought from Millipore (Burlington, MA). Human A549 lung carcinoma cell line and F-12K medium were obtained from ATCC, Manassas, VA. Hank’s balanced salt solution, 0.05% trypsin, and antibiotic-antimycotic mixture were bought from Mediatech (Washington, DC). Fetal bovine serum (FBS) was obtained from Atlas Biologicals, Fort Collins, CO. ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit was purchased from BPS Bioscience, San Diego, CA. Anti-human IL-6 antibody and human IL-1β and IL-6 ELISA kits were bought from ThermoFisher, Waltham, MA.
Vsv g pseudotyped particles
Manufactured by GeneCopoeia
VSV-G-pseudotyped particles are a type of viral vector used for gene delivery. They are engineered to incorporate the vesicular stomatitis virus glycoprotein (VSV-G) on their surface, which facilitates their entry into a wide range of cell types. These particles can be used to deliver genetic material to target cells for various applications, such as gene expression studies or gene therapy.
Automatically generated - may contain errors
Lab products found in correlation
Sars cov 2 spike pseudotyped lentiviral particles, by GeneCopoeia (2 mentions)
Human ace2 expressing hek293 cells, by GeneCopoeia (2 mentions)
Human a549 lung carcinoma cell line, by American Type Culture Collection (2 mentions)
Ace2 sars cov 2 spike inhibitor screening assay kit, by BPS Biosciences (2 mentions)
F 12k medium, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Atlas Biologicals (2 mentions)
Anti sars cov 2 spike s1 antibody, by Abcam (1 mentions)
Oleanolic acid, by Merck Group (1 mentions)
Eugenol, by Merck Group (1 mentions)
Ursolic acid, by Merck Group (1 mentions)
Mouse tnf α elisa kit, by Thermo Fisher Scientific (1 mentions)
Recombinant human ace2 protein 18 739, by MyBioSource (1 mentions)
Methylcellulose, by Spectrum Chemical (1 mentions)
2 protocols using vsv g pseudotyped particles
1
SARS-CoV-2 Spike Protein Inhibition Assay
2
Screening Inhibitors of SARS-CoV-2 Spike-ACE2 Interaction
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Eugenol, ursolic acid, oleanolic acid, and β-caryophylline were purchased from Sigma, St. Louis, MO. Methyl cellulose was bought from Spectrum Chemical, Gardena, CA. SARS-CoV-2 spike-pseudotyped lentiviral particles, VSV-G-pseudotyped particles and human ACE2-expressing HEK293 cells were purchased from GeneCopoeia, Rockville, MD. Recombinant COVID-19 Spike protein S1 (14-685) was purchased from Abeomics, San Diego, CA. Recombinant human ACE2 protein (18-739) was purchased from MyBiosource, San Diego, CA. Human A549 lung carcinoma cell line and F-12 K medium were obtained from ATCC, Manassas, VA. Hank’s balanced salt solution, 0.05 % trypsin, and antibiotic-antimycotic mixture were bought from Mediatech (Washington, DC). Fetal bovine serum (FBS) was obtained from Atlas Biologicals, Fort Collins, CO. ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit was purchased from BPS Bioscience, San Diego, CA. Mouse TNFα ELISA kit was bought from ThermoFisher, Waltham, MA.
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