Fxa epiflourescence microscope

Manufactured by Nikon

The FXA epiflourescence microscope is a laboratory equipment designed for fluorescence microscopy. It provides high-quality imaging of fluorescently labeled samples, allowing for the visualization and analysis of cellular structures, proteins, and other biological entities.

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Lab products found in correlation

Vectashield, by Vector Laboratories (2 mentions) Odyssey blocking buffer, by LI COR (1 mentions)

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Nikon-FXA microscope (7 citations)

2 protocols using fxa epiflourescence microscope

Immunohistochemical Analysis of AGT in Kidney

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Kidneys were fixed overnight in 10% formaldehyde, embedded in paraffin and 4 μm sections obtained. Kidney sections were rehydrated with xylene and ethanol and treated with 1% SDS for 10 min to enhance antibody staining. After blocking with 1% BSA in PBS for 1 hr, sections were incubated with primary antibody against AGT (1:25, IBL America, Minneapolis, MN) overnight. After three consecutive washes of 5 min each with PBS, kidney sections were incubated with secondary donkey anti goat Alexa Fluor 488 (1:50) antibodies for 60 min. After three wash‐rinse steps of 5 min each with PBS, slides were mounted in Vectashield (Vector Laboratories, Burlingame, CA) and sealed with a coverslip. Tissue sections were examined and photographed with a Nikon FXA epiflourescence microscope.

Ramkumar N., Stuart D., Calquin M., Wang S., Niimura F., Matsusaka T, & Kohan D.E. (2016). Possible role for nephron‐derived angiotensinogen in angiotensin‐II dependent hypertension. Physiological Reports, 4(1), e12675.

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Immunofluorescent Localization of Kidney Proteins

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Kidneys from control and PRR KO mice were fixed overnight in 10 % formaldehyde, embedded in paraffin and 4 μm sections obtained. Deparaffinized kidney sections were treated with 0.1 % Triton for 10 min for antigen retrieval, blocked with Odyssey blocking buffer (Licor, Lincoln, NE, USA) for 1 h and incubated with primary antibody against ETA (1:25, Alomone, Jerusalem, Israel) and AQP2 (1:200, Santa Cruz, Dallas, TX, USA) overnight. After 3 consecutive washes of 5 min each with phosphate-buffered saline (PBS), kidney sections were incubated with secondary donkey anti- rabbit Alexa Fluor 488 (1:200) and donkey anti-goat Alexa Fluor 555 (1:400) antibodies for 60 min. After 3 wash-rinse steps of 5 min each with PBS, slides were mounted in Vectashield (Vector Laboratories, Burlingame, CA, USA) and sealed with a coverslip. Tissue sections were examined and photographed with a Nikon FXA epiflourescence microscope.

RAMKUMAR N., STUART D., ABRAHAM N, & KOHAN D.E. (2018). Nephron Prorenin Receptor Deficiency Alters Renal Medullary Endothelin-1 and Endothelin Receptor Expression. Physiological research, 67(SUPPLEMENTUM 1), S127-S136.

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