Gipz human huwe1 shrna

Manufactured by Thermo Fisher Scientific

The GIPZ Human HUWE1 shRNA is a lentiviral vector that expresses a short hairpin RNA (shRNA) targeting the HUWE1 gene in human cells. The HUWE1 gene encodes a large multi-domain E3 ubiquitin-protein ligase that plays a role in various cellular processes. The GIPZ vector provides a tool for researchers to study the function of the HUWE1 gene through RNA interference-mediated knockdown.

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Pgipz ns, by Thermo Fisher Scientific (2 mentions)

2 protocols using gipz human huwe1 shrna

Lentiviral Transduction of mule-targeting shRNA

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Cells from male control were lentivirally transduced with plasmids carrying the non-specific control shRNA (pGIPZ-Ns, Open Biosystems) or the mule-targeting shRNA (GIPZ Human HUWE1 shRNA, Clone-ID V3LHS_353153, Open Biosystems). Stable clones were subsequently selected with puromycin, while the selection efficiency was monitored by the percentage of green fluorescent protein (GFP)-positive cells.

Friez M.J., Brooks S.S., Stevenson R.E., Field M., Basehore M.J., Adès L.C., Sebold C., McGee S., Saxon S., Skinner C., Craig M.E., Murray L., Simensen R.J., Yap Y.Y., Shaw M.A., Gardner A., Corbett M., Kumar R., Bosshard M., van Loon B., Tarpey P.S., Abidi F., Gecz J, & Schwartz C.E. (2016). HUWE1 mutations in Juberg-Marsidi and Brooks syndromes: the results of an X-chromosome exome sequencing study. BMJ Open, 6(4), e009537.

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Genetic Manipulation of Mule in LCLs

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Wild-type (WT) LCLs were lentivirally transduced with plasmids carrying the non-specific control shRNA (pGIPZ-Ns, Open Biosystems) or the Mule-targeting shRNA (GIPZ Human HUWE1 shRNA, Clone-ID V3LHS_353153, TCCTCTGTACCAACAACCT, Open Biosystems). Stable clones were subsequently selected with puromycin (1 µg/ml), while the selection-efficiency was monitored by the percentage of GFP-positive cells.

Bosshard M., Aprigliano R., Gattiker C., Palibrk V., Markkanen E., Backe P.H., Pellegrino S., Raymond F.L., Froyen G., Altmeyer M., Bjørås M., Dianov G.L, & van Loon B. (2017). Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability. Scientific Reports, 7, 15050.

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