The procedure for PCR quantification was described previously (9 (link)–11 (link)). TaqMan probes and primers for LOXL2 (P/N: Hs00158757_m1; Applied Biosystems, Foster City, CA, USA) were assay-on-demand gene expression products. Stem-loop RT-PCRs for miR-29a (P/N: 002112; Applied Biosystems), miR-29b (P/N: 000413), and miR-29c (P/N: 000587) were used to quantify the expression levels of miRNAs according to the manufacturer's protocol. To normalize the data for quantification of mRNA and miRNAs, we used human GUSB (P/N: Hs99999908_m1; Applied Biosystems) and RNU48 (P/N: 001006; Applied Biosystems), respectively.
Taqman probes and primers for loxl2
Manufactured by Thermo Fisher Scientific
Sourced in United States
TaqMan probes and primers for LOXL2 are molecular biology tools designed for the detection and quantification of the lysyl oxidase-like 2 (LOXL2) gene. These products provide a specific and sensitive method for analyzing LOXL2 expression levels in research samples.
Automatically generated - may contain errors
3 protocols using taqman probes and primers for loxl2
1
Quantification of LOXL2 and miR-29 family
2
PCR Quantification of LOXL2, PLOD2, and miRNA
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The procedure for PCR quantification was previously described. TaqMan probes and primers for LOXL2 (P/N: Hs00158757_ml; Applied Biosystems, Foster City, CA, USA), PLOD2 (P/N: Hs01118190_ml; Applied Biosystems) and GUSB (the internal control; P/N: Hs00939627_ml; Applied Biosystems) were assay-on-demand gene expression products. The expression levels of miR-26a (assay ID: 000405; Applied Biosystems) and miR-26b (assay ID: 000407; Applied Biosystems) were analyzed by TaqMan quantitative real-time RT-PCR (TaqMan MicroRNA assay; Applied Biosystems) and normalized to the expression of RNU48 as previously described (12 (link),20 (link),21 (link)).
3
Profiling miRNA and mRNA Expressions
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The expression levels of miR-26a (Assay ID: 000405), miR-26b (Assay ID: 000407), miR-29a (Assay ID: 002112), miR-29b (Assay ID: 000413), miR-29c (Assay ID: 000413) and miR-218 (Assay ID: 000587) were analyzed by TaqMan qRT-PCR and normalized to RNU48 (Assay ID: 001006). TaqMan probes and primers for LOXL2 (P/N: Hs00158757_m1; Applied Biosystems, Foster City, CA, USA), LOX (P/N: Hs00942480_m1) and GUSB (P/N: Hs99999908_ml) as an internal control were obtained from Applied Biosystems (Assay-On-Demand Gene Expression Products).
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