S epidermidis

Manufactured by RIKEN BioResource Center

Sourced in Japan

S. epidermidis is a bacterial strain commonly found on the human skin. It serves as a model organism for studying the biology and interactions of staphylococcal species.

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Lab products found in correlation

Shaking incubator, by Thermo Fisher Scientific (1 mentions) S aureus, by RIKEN BioResource Center (1 mentions) E coli, by RIKEN BioResource Center (1 mentions) Mrs agar, by Merck Group (1 mentions)

2 protocols using s epidermidis

Microbial Growth and Strain Culturing

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Microbial media and growth plates were prepared prior to growing
various microbe strains. Sterilized Trypticase Soy Broth (TSB) (Merck
Co., Ltd.) and de Man, Rogosa, Sharpe (MRS) (Merck Co., Ltd.) broth
were used for all microbe cultures used in this study. Similarly,
sterilized Trypticase Soy Agar (TSA) (Merck Co., Ltd.) and MRS agar
(Merck Co., Ltd.) were poured and solidified in 100 × 15 mm petri
dishes.
Cultures of L. crispatus (Bioresource Collection and Research Center, Hsinchu, Taiwan) were
grown in MRS broth; on the other hand, MSSA, S. aureus, S. epidermidis, E.
faecalis, S. agalactiae, S. pneumoniae, E.
coli, K. pneumoniae, and C. albicans (Bioresource Collection
and Research Center) were grown in TSB. All cultures were prepared
in a shaking incubator (Thermo Fisher Scientific) set at 37 °C
and 200 rpm for 24 h. The cultures were diluted to a 0.5 MacFarland
bacterial turbidity standard using a UV–VIS optical density
spectrophotometer (Vernier Software & Technology, Beaverton, OR,
USA). 50 μL of different microbes was added to their corresponding
nutrient plate, and sterilized cell spreaders were used to equally
distribute the microbes on the plate.

Wang W.B., Pan C.Y., Huang E.Y., Peng B.J., Hsu J, & Clapper J.C. (2022). Electrospun Polyacrylonitrile Silver(I,III) Oxide Nanoparticle Nanocomposites as Alternative Antimicrobial Materials. ACS Omega, 7(51), 48173-48183.

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Bacterial Culture Preparation Protocol

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The lysogeny broth (LB) was composed of 2 g of Bacto Tryptone, 1 g of Bacto yeast extract, and 2 g of NaCl in 200 mL of distilled water. S. aureus IAM1011, S. aureus ATCC25923, S. aureus ATCC29213, S. pseudintermedius 2012-S-27, S. epidermidis ATCC12228, Enterococcus faecalis ATCC29212, E. coli K12W3110, E. coli ATCC25922, Pseudomonas aeruginosa ATCC27853, and Salmonella enteritidis ATCC13311 were provided by RIKEN BRC (Ibaraki, Japan). All bacteria were cultured at 37 °C overnight on LB agar plates. A mixture of 200 mL of LB and 3 g of agar was used to prepare each LB plate. Cultured colonies were selected and isolated overnight in LB at 37 °C. The suspension was centrifuged (10,000 rpm, 1 min) and washed twice with distilled water. The corresponding buffer was added to the washed cells, and the bacterial suspension was centrifuged. The concentration of the bacterial suspension was adjusted by measuring the optical density at 600 nm (OD₆₀₀) after vortex mixing. The 4.5 × 10⁸ CFU·mL⁻¹S. aureus IAM1011 suspension gave OD₆₀₀ = 1.0, and the 1.0 × 10⁹ CFU·mL⁻¹ E. coli K12W3110 suspension gave OD₆₀₀ = 1.0. The generated bacterial cultures were used in the following experiments.

Mikagi A., Takahashi Y., Kanzawa N., Suzuki Y., Tsuchido Y., Hashimoto T, & Hayashita T. (2023). Aggregation-Based Bacterial Separation with Gram-Positive Selectivity by Using a Benzoxaborole-Modified Dendrimer. Molecules, 28(4), 1704.

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