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4 amino 5 4 chlorophenyl 7 t butyl pyrazolo 3 4 d pyrimidine pp2

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4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo[3,4-d] pyrimidine (PP2) is a chemical compound that functions as a selective inhibitor of Src family kinases.

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Lab products found in correlation

Orientin, by Merck Group (1 mentions) Ly294002, by Cell Signaling Technology (1 mentions) Phosphor p38, by Cell Signaling Technology (1 mentions) Sp600125, by Cell Signaling Technology (1 mentions) Phospho src, by Cell Signaling Technology (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Fluorophore labeled secondary antibodies, by LI COR (1 mentions) U0126, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Sb203580, by Cell Signaling Technology (1 mentions) N acetyl l cysteine, by Merck Group (1 mentions) Deadendtm fluorometric tunel system, by Promega (1 mentions) Lipofectaminetm rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Mouse cytokine array panel a, by R&D Systems (1 mentions) Maleimide, by Merck Group (1 mentions) U46619, by Merck Group (1 mentions) Anti mouse igg, by Merck Group (1 mentions) Anti arhgef1, by Cell Signaling Technology (1 mentions)

4 protocols using 4 amino 5 4 chlorophenyl 7 t butyl pyrazolo 3 4 d pyrimidine pp2

1

Signaling Pathway Inhibitors in Cell Assays

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Orientin, H2O2, N-acetyl-L-cysteine (NAC), 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo[3,4-d] pyrimidine (PP2), and 4-amino-7-phenylpyrazol [3,4-d] pyrimidine (PP3) were purchased from Sigma-Aldrich (St Louis, MO, USA). Antibodies specifically targeted to caspase-3, PARP, GAPDH, JNK, p38, ERK, AKT, SRC, phospho-JNK, phosphor-p38, phospho-ERK, phospho-AKT, and phospho-Src were purchased from Cell Signaling Technology (Danvers, MA, USA). Fluorophore-labeled secondary antibodies were purchased from LI-COR Biosciences (Lincoln, NE, USA). Inhibitors of ERK, p38, JNK, and AKT namely U0126, SB203580, SP600125, and LY294002, respectively, were purchased from Cell Signaling Technology (Danvers, MA, USA).
Qi S., Feng Z., Li Q., Qi Z, & Zhang Y. (2018). Inhibition of ROS-mediated activation Src-MAPK/AKT signaling by orientin alleviates H2O2-induced apoptosis in PC12 cells. Drug Design, Development and Therapy, 12, 3973-3984.
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2

Signaling Pathways in Cytokine-Induced Apoptosis

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Src kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo[3,4-d]pyrimidine (PP2) was purchased from Sigma-Aldrich (St. Louis, MO, United States). Recombinant mice tumor necrosis factor-α (TNF-α) (315-01A), interferon-γ (IFN-γ) (500-P119) was purchased from PeproTech (Rocky Hill, NJ, United States). Fas (sc-2931) and Fap-1 (sc-145067) small interfering RNA (siRNA) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States). LipofectamineTM RNAiMAX transfection reagent (#13778030) was purchased from Thermo Fisher Scientific (Waltham, MA, United States). Mouse Cytokine Array Panel A (ARY006) was obtained from R&D system (Minneapolis, MN, United States). Mouse CXCL10 enzyme-linked immunosorbent assay (ELISA) Kit (ab214563) was purchased from Abcam (Cambridge, MA, United States). DeadEndTM Fluorometric TUNEL System (G3250) was purchased from Promega (Madison, WI, United States).
Wang P., Zhao Y., Wang J., Wu Z., Sui B., Mao X., Shi S, & Kou X. (2021). Dephosphorylation of Caveolin-1 Controls C-X-C Motif Chemokine Ligand 10 Secretion in Mesenchymal Stem Cells to Regulate the Process of Wound Healing. Frontiers in Cell and Developmental Biology, 9, 725630.
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3

Investigating Src-family Kinase Inhibitors

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The Src-family kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2), the antioxidants ebselen and hydroxy-tempo (Tempol), the intracellular superoxide generator 6-anilo-5,8-quinoledione (LY83583) and the thromboxane A2 mimetic U46619 were all obtained from Merck Millipore. Superoxide dismutase (SOD), catalase, maleimide, polyethyleneglycol maleimide 5000, dithiothreitol, the nitric oxide synthase inhibitor L-nitro-arginine methyl-ester (L-NAME) and the inhibitor of RGS-domain containing RhoGEFs (Y16) were obtained from Sigma. Anti-Src, anti-phospho-Src family kinases (Tyr416), anti-MLC20, anti-phospho-MLC20 (Ser19), anti-MYPT1, anti-ARHGEF1, anti-phospho-tyrosine (PY100) and anti-GAPDH were all obtained from Cell Signalling (UK). Anti-phospho-MYPT1 (Thr850) was from Upstate (UK). Anti-mouse IgG and anti-rabbit IgG were from Sigma.
MacKay C.E., Shaifta Y., Snetkov V.V., Francois A.A., Ward J.P, & Knock G.A. (2017). ROS-dependent activation of RhoA/Rho-kinase in pulmonary artery: Role of Src-family kinases and ARHGEF1. Free Radical Biology & Medicine, 110, 316-331.
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4

Peptide-Based aPKC Inhibition Assay

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aPKC pseudosubstrate peptide (Myr-SIYRRGARRWRKL(biotin)YCAN), scrambled aPKC (Myr-WRICGNKARL(biotin)RRYYSAR, and PKCα/β (Myr-RFARKGAL(biotin)RQKNVHEVKN) were synthesized by GenScript Corp. (Piscataway, NJ). aPKCι cDNA was generously provided by Dr. Trevor Biden (Garvan Institute of Medical Research, Sydney, Australia). The mouse embryo fibroblast cell line SYF was purchased from American Type Culture Collection (Manassas, VA). Primary and secondary antibodies were purchased from the indicated suppliers; anti-PKC from BD Biosciences (San Jose, CA); anti-Src and anti-phospho-Src (Tyr416) from Cell Signaling Technology, Inc. (Danvers, MA); Alexa Fluor 488 chicken antimouse antibody, Alexa Fluor 594 chicken anti-rabbit, and Hoechst 33342 from Life Technologies (Grand Island, NY). FITC-streptavidin was purchased from ThermoScientific (Pittsburgh, PA). 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo(3,4-d)pyrimidine (PP2) was purchased from EMD Millipore (Billerica, MA). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Tisdale E.J., Shisheva A, & Artalejo C.R. (2014). Overexpression of Atypical Protein Kinase C in HeLa Cells Facilitates Macropinocytosis via Src Activation. Cellular signalling, 26(6), 1235-1242.
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