Protease free bovine serum albumin

Manufactured by Merck Group

Protease-free bovine serum albumin is a purified protein derived from bovine serum. It is used as a component in various cell culture media and buffer solutions to support cell growth and maintain cellular functions.

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Lab products found in correlation

Normal donkey serum (nds), by Abcam (1 mentions) Paraformaldehyde (pfa), by Santa Cruz Biotechnology (1 mentions) Prolong gold mounting medium with dapi, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions) Rat anti cd45 fitc, by BioLegend (1 mentions) Rat anti cd49f pe, by BioLegend (1 mentions) Rat anti cd326 epcam apc cy7, by BioLegend (1 mentions) Goat anti trop2 apc, by R&D Systems (1 mentions) Trustain fcx anti mouse cd16 32 antibody, by BioLegend (1 mentions) Sodium azide, by Merck Group (1 mentions) Facscanto flow cytometer, by BD (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Enhanced chemiluminescence, by Merck Group (1 mentions) Non fat milk, by Bio-Rad (1 mentions) Bis tris precast gel, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Bovine albumin (95 citations) Bovine serum (80 citations) -free bovine serum albumin (8 citations) Serum bovine albumin (7 citations) Albumins ( (2 citations)

3 protocols using protease free bovine serum albumin

Immunohistochemical Analysis of Collagen Types

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hADMs were snap-frozen after coincubation with PBMCs mentioned previously. hADMs were cut using cryomicrotome into 20 μM longitudinal sections and seeded on glass slides. Next, cryosections were fixed with 4% paraformaldehyde (Santa Cruz Biotechnology, Dallas, TX, USA) and incubated in a detergent (0.1% Triton X-100 (Sigma)) in 0.02% SDS-PBS (Sigma-Aldrich and Corning, respectively), followed by incubation with blocking buffer (10% normal donkey serum–Abcam, Cambridge, UK) in 1% BSA in PBS). Next, the slides were stained with a specific primary antibody for collagen types I, III and IV or incubated in staining buffer (1% protease-free bovine serum albumin (Sigma) in PBS) for 60 min in a high humidity chamber in the dark. Next, the slides were washed three times in washing buffer (Tween20-PBS) and stained with appropriate secondary antibodies. For detailed characteristics of primary and related secondary antibodies, please see Supplementary Table S3. Finally, the specimens were mounted in Prolong Gold mounting medium with DAPI (Thermo Fisher) and covered with cover slides (Avantor, Gliwice, Poland), followed by overnight incubation at RT in the dark before analysis by confocal microscopy.

Holl J., Pawlukianiec C., Corton Ruiz J., Groth D., Grubczak K., Hady H.R., Dadan J., Reszec J., Czaban S., Kowalewski C., Moniuszko M, & Eljaszewicz A. (2021). Skin Substitute Preparation Method Induces Immunomodulatory Changes in Co-Incubated Cells through Collagen Modification. Pharmaceutics, 13(12), 2164.

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Prostate Cell Immunophenotyping by Flow

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Single-cell suspensions of 5 × 10⁴–1 × 10⁶ cells from mouse prostate tissue of different ages (each n = 4) were stained in cell staining buffer comprised of 1X DPBS (Gibco) with 5 g/L protease-free bovine serum albumin (Sigma-Aldrich) and 200 mg/L sodium azide (Sigma-Aldrich). Nonspecific antibody binding by Fc receptors was blocked using TruStain fcX (anti-mouse CD16/32) Antibody (BioLegend) according to the manufacturer’s protocol. Cells were then stained with rat anti-CD49f-PE (BioLegend), rat anti-CD326 (EpCAM)-APC/Cy7 (BioLegend), goat anti-Trop2-APC (R&D Systems), and rat anti-CD45-FITC (BioLegend) for 30 minutes at room temperature. Cells were washed with cell staining buffer then fixed in 1% paraformaldehyde (Electron Microscopy Sciences) for 10–15 minutes at 37°C. After fixation, cells were chilled on ice for 1 minute, washed with cell staining buffer, and stored at 4°C before analysis on a FACSCanto flow cytometer (BD Biosciences).

Fox J.J., Hashimoto T., Navarro H.I., Garcia A.J., Shou B.L, & Goldstein A.S. (2023). Highly multiplexed immune profiling throughout adulthood reveals kinetics of lymphocyte infiltration in the aging mouse prostate. Aging (Albany NY), 15(9), 3356-3380.

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Protein Extraction and Western Blot Analysis

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Tumor tissues (50–75 mg/mouse) were minced on ice and homogenized using a Dounce homogenizer and centrifuged at 16,000g at 4°C for 10 min. The total protein in samples was determined using the Pierce Protein Assay kit. Fifty micrograms of sample was electrophoresed on 4–12% Bis-Tris precast gels (Life Technologies, Carlsbad, CA). After electrotransfer to nitrocellulose, membranes were blocked at room temperature with TBST [10 mmol/L Tris-HCl (pH 7.5), 0.5 mol/L NaCl and 0.1% (v/v) Tween 20] containing 5% nonfat milk (BioRad, Hercules, CA) for 1 hr. Cleaved Notch, cleaved caspase 3 and actin primary antibodies (Cell Signaling Technologies, Danvers, MA) were diluted at 1:1,000 in TBST containing 5% protease-free bovine serum albumin (Sigma-Aldrich, St. Louis, MO), and the membranes were incubated overnight at 4°C with rocking. After washing three times with TBST, the membranes were incubated for 1 hr at room temperature with anti-rabbit IgG horseradish peroxidase-conjugated antibody at a final dilution of 1:50,000 in TBST. After washing three times with TBST, bound antibodies were detected by enhanced chemiluminescence (Millipore, Temecula, California).

Arcaroli J.J., Tai W.M., McWilliams R., Bagby S., Blatchford P.J., Varella-Garcia M., Purkey A., Quackenbush K.S., Song E.K., Pitts T.M., Gao D., Lieu C., McManus M., Tan A.C., Zheng X., Zhang Q., Ozeck M., Olson P., Jiang Z.Q., Kopetz S., Jimeno A., Keysar S., Eckhardt G, & Messersmith W.A. (2015). A NOTCH1 gene copy number gain is a prognostic indicator of worse survival and a predictive biomarker to a Notch1 targeting antibody in colorectal cancer. International journal of cancer. Journal international du cancer, 138(1), 195-205.

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