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Anti myc clone y69

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Anti-MYC (clone Y69) is a recombinant rabbit monoclonal antibody that recognizes the c-Myc protein. It can be used in various applications such as Western blotting, immunohistochemistry, and immunocytochemistry to detect the c-Myc protein.

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Lab products found in correlation

Anti tubulin beta3 clone tuj1, by BioLegend (2 mentions) Ultraview dab, by Roche (2 mentions) Ecl reagent, by Cytiva (1 mentions) Anti gapdh clone 6c5, by Merck Group (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions) Biotinylated anti rabbit igg, by Vector Laboratories (1 mentions) Anti rabbit igg alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Irdye 680rd anti mouse igg, by LI COR (1 mentions) Anti gapdh clone 14c10, by Cell Signaling Technology (1 mentions) Irdye 800cw anti rabbit igg, by LI COR (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Dmi6000b microscope, by Leica (1 mentions)

Spelling variants of this product

Anti-Myc (113 citations) Myc (clone Y69; (13 citations) MYC (Y69) (8 citations) Clone Y69 (4 citations) Anti-MYC (Y69, (3 citations)

4 protocols using anti myc clone y69

1

Quantitative Immunostaining of TUJ1 and MYC

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Double labeling immunohistochemistry was performed using a 1:8000 dilution of anti-tubulin beta3 (clone TUJ1, Biolegend) and 1:25 dilution of anti-MYC (clone Y69, Abcam) diluted in Ventana antibody diluent (Roche Tissue Diagnostic, 251–018) and detected using the UltraView Red (Roche Tissue Diagnostics, 760–501) and UltraView DAB (Roche Tissue Diagnostics, 760–500) detection kits, respectively. Each target was evaluated using a semi-quantitative system to construct an H-score, obtained by multiplying the intensity of the stain (0: no staining; 1: weak staining; 2: moderate staining, and 3: strong staining) by the percentage (0 to 100) of cells showing that staining intensity (H-score range, 0 to 300).
Hovestadt V., Smith K.S., Bihannic L., Filbin M.G., Shaw M.L., Baumgartner A., DeWitt J.C., Groves A., Mayr L., Weisman H.R., Richman A.R., Shore M.E., Goumnerova L., Rosencrance C., Carter R.A., Phoenix T.N., Hadley J.L., Tong Y., Houston J., Ashmun R.A., DeCuypere M., Sharma T., Flasch D., Silkov A., Ligon K., Pomeroy S.L., Rivera M.N., Rozenblatt-Rosen O., Rusert J.M., Wechsler-Reya R.J., Li X.N., Peyrl A., Gojo J., Kirchhofer D., Lötsch D., Czech T., Dorfer C., Haberler C., Geyeregger R., Halfmann A., Gawad C., Easton J., Pfister S.M., Regev A., Gajjar A., Orr B.A., Slavc I., Robinson G.W., Bernstein B.E., Suvà M.L, & Northcott P.A. (2019). Resolving medulloblastoma cellular architecture by single-cell genomics. Nature, 572(7767), 74-79.
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2

Liver Protein Extraction and Analysis

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Livers were homogenized in the lysis buffer containing 50 mM HEPES-KOH pH 7.4, 130 mM NaCl, 1% Triton X-100, 1% SDS, 1% sodium deoxycholate, and complete protease inhibitor cocktail (Roche). Lysates were boiled for 5 min in Laemmli buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 2% SDS, 0.01% bromophenol blue, and 100 mM DTT), and proteins were electrophoresed and immunoblotted. Blots were probed with anti-MYC (clone Y69, Abcam) and anti-GAPDH (clone 6C5, EMD Millipore), followed by HRP-conjugated secondary antibodies and ECL reagent (Amersham).
Juric V., Ruffell B., Evason K.J., Hu J., Che L., Wang L., Chen X, & Bishop J.M. (2015). Monocyte-dependent liver injury promotes carcinogenesis in an oncogene-specific manner. Journal of hepatology, 64(4), 881-890.
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3

Western Blot and Immunostaining Antibody Panel

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The following antibodies were used for Western blot (WB) anlysis: Anti-MYC clone 9E10 (1:5000, Millipore-Sigma, M4439), Anti-MYC clone Y69 (1:1000, Abcam, Ab32072), Anti-Exportin-1 clone D6V7N (1:1000, Cell Signaling Technology, 46249), Anti-GAPDH clone 14C10 (1:5000, Cell Signaling Technology, 2118), Anti-alpha Tubulin clone DM1A (1:2000, Millipore-Sigma, T9026), Anti-beta Actin (1:1000, Cell Signaling Technology, 4967), Anti-rabbit IgG IRDye800CW (1:10000, Licor, 926-32211), Anti-mouse IgG IRDye680RD (1:10000, Licor, 926-68070), Anti-rabbit IgG-AP (1:5000, Invitrogen, G-21079), Anti-mouse IgG/IgM-AP (1:5000, Invitrogen, 31330). The following antibodies were used for immunohistochemistry (IHC): Anti-MYC clone EP121 (1:150, Millipore-Sigma, 395 R), Anti-cleaved Caspase-3 (Asp175) (1:100, Cell Signaling Technology, 9661), Biotinylated anti-rabbit IgG (1:500, Vector Laboratories, BA-1000-1.5). For immunofluorescence (IF) stainings, we used the following antibodies: Anti-phospho Histone-3 (Ser10) (1:200, Cell Signaling Technology, 9701), Anti-cleaved Caspase-3 (Asp175) (1:100, Cell Signaling Technology, 9661), Anti-rabbit IgG AlexaFluor568 (1:400, Invitrogen, A-11011). Immunofluorescence or bright field images were taken with 20x objectives on a Leica DMI6000 B microscope and quantified using MetaMorph (v7.8) image analysis software.
Deutzmann A., Sullivan D.K., Dhanasekaran R., Li W., Chen X., Tong L., Mahauad-Fernandez W.D., Bell J., Mosley A., Koehler A.N., Li Y, & Felsher D.W. (2024). Nuclear to cytoplasmic transport is a druggable dependency in MYC-driven hepatocellular carcinoma. Nature Communications, 15, 963.
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4

Quantitative Immunostaining of TUJ1 and MYC

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Double labeling immunohistochemistry was performed using a 1:8000 dilution of anti-tubulin beta3 (clone TUJ1, Biolegend) and 1:25 dilution of anti-MYC (clone Y69, Abcam) diluted in Ventana antibody diluent (Roche Tissue Diagnostic, 251–018) and detected using the UltraView Red (Roche Tissue Diagnostics, 760–501) and UltraView DAB (Roche Tissue Diagnostics, 760–500) detection kits, respectively. Each target was evaluated using a semi-quantitative system to construct an H-score, obtained by multiplying the intensity of the stain (0: no staining; 1: weak staining; 2: moderate staining, and 3: strong staining) by the percentage (0 to 100) of cells showing that staining intensity (H-score range, 0 to 300).
Hovestadt V., Smith K.S., Bihannic L., Filbin M.G., Shaw M.L., Baumgartner A., DeWitt J.C., Groves A., Mayr L., Weisman H.R., Richman A.R., Shore M.E., Goumnerova L., Rosencrance C., Carter R.A., Phoenix T.N., Hadley J.L., Tong Y., Houston J., Ashmun R.A., DeCuypere M., Sharma T., Flasch D., Silkov A., Ligon K., Pomeroy S.L., Rivera M.N., Rozenblatt-Rosen O., Rusert J.M., Wechsler-Reya R.J., Li X.N., Peyrl A., Gojo J., Kirchhofer D., Lötsch D., Czech T., Dorfer C., Haberler C., Geyeregger R., Halfmann A., Gawad C., Easton J., Pfister S.M., Regev A., Gajjar A., Orr B.A., Slavc I., Robinson G.W., Bernstein B.E., Suvà M.L, & Northcott P.A. (2019). Resolving medulloblastoma cellular architecture by single-cell genomics. Nature, 572(7767), 74-79.
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