Immunohistochemistry was performed as described previously [22 (link)] on 10 µm frozen sections of cold phosphate buffered saline (PBS) perfused spinal cords taken from mice subject to either normoxia (control) or hypoxic conditions. The following antibodies were used in this study: rat monoclonal antibodies reactive for CD31 (clone MEC13.3) and the integrin subunit α5 (clone 5H10-27 (MFR5)) (BD Pharmingen (La Jolla, CA), hamster anti-CD31 (clone 2H8, Abcam, Cambridge, MA), rabbit anti-fibronectin and anti-laminin (Sigma, St. Louis, MO), mouse anti-α-SMA-Cy3 conjugate (Sigma, clone 1A4), rabbit anti-claudin-5, rabbit anti-occludin and rabbit anti-ZO-1) (all from Invitrogen, Carlsbad, CA), and mouse anti-Ki67 (Vector laboratories, Burlingame, CA). Secondary antibodies used included goat anti-rabbit Cy3, goat anti-rat Cy3, goat anti-mouse Cy3, and goat anti-rabbit Cy5 (far red) (all from Jackson Immunoresearch, Baltimore, PA) and anti-rat Alexa Fluor 488 and anti-hamster Alexa 488 (both from Invitrogen).
Anti hamster alexa 488
Manufactured by Thermo Fisher Scientific
The Anti-hamster Alexa 488 is a fluorescently labeled antibody that targets hamster proteins. It is designed for use in various immunodetection techniques, such as flow cytometry, immunohistochemistry, and western blotting, to identify and quantify hamster-derived biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti mouse cy3, by Jackson ImmunoResearch (1 mentions)
Mouse anti ki67, by Vector Laboratories (1 mentions)
Rabbit anti occludin, by Thermo Fisher Scientific (1 mentions)
Anti rat alexafluor488, by Thermo Fisher Scientific (1 mentions)
Goat anti rat cy3, by Jackson ImmunoResearch (1 mentions)
Rabbit anti claudin 5, by Thermo Fisher Scientific (1 mentions)
Hamster anti cd31 clone 2h8, by Abcam (1 mentions)
Rabbit anti zo 1, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit cy3, by Jackson ImmunoResearch (1 mentions)
Collagenase 4, by Thermo Fisher Scientific (1 mentions)
Fortessa analyzer, by BD (1 mentions)
Rat anti cd45 apc cy7, by BioLegend (1 mentions)
Rat anti cd31 apc, by BD (1 mentions)
2 protocols using anti hamster alexa 488
1
Immunohistochemical Profiling of Spinal Cord
2
Multicolor Flow Cytometry of Ear Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ears were digested with collagenase IV (Invitrogen) as described [19 (link)]. Ear single cell suspensions were stained with the following antibodies: rat anti-CD45-APC-Cy7 (1:250, Biolegend), rat anti-CD31-APC (1:100, BD), hamster anti-podoplanin (1:100, clone 8.1.1, Developmental Studies Hybridoma Bank, University of Iowa) and anti-hamster-Alexa 488 (1:200, Invitrogen). FACS analysis was performed on a BD Fortessa analyzer (BD Biosciences) using the FACSDiva software. Data were analyzed with FlowJo software (TreeStar).
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