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Luc screen system

Manufactured by Thermo Fisher Scientific
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The Luc-screen system is a laboratory equipment designed for luminescence-based detection and analysis. It provides a platform for sensitive and quantitative measurement of luciferase-based reporter gene expression in various biological samples. The core function of the Luc-screen system is to enable reliable and reproducible luminescence detection to support research and experimental workflows.

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Lab products found in correlation

Lps from e coli, by Merck Group (2 mentions)

Close spelling variants of this product

Luc-screen Firefly Luciferase Gene Reporter System (2 citations) Luc-Screen Extended-Glow Luciferase Reporter Gene Assay system (2 citations)

2 protocols using luc screen system

1

NF-κB Activation Assay in RAW264.7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
RAW264.7 cells, stably transfected with the 3x-κBluc plasmid9 (link), were incubated with the small molecules (10 μM) or vehicle for 1 h. Subsequently, cells were activated using (LPS) from E. coli (Sigma-Aldrich). After 2 h, cells were lysed, and substrate was added according to the manufacturer’s protocol (Luc-screen system; Applied Biosystems). Emission was measured at 450 nm using the Wallac Victor II luminometer.
Lameijer M., Binderup T., van Leent M.M., Senders M.L., Fay F., Malkus J., Sanchez-Gaytan B.L., Teunissen A.J., Karakatsanis N., Robson P., Zhou X., Ye Y., Wojtkiewicz G., Tang J., Seijkens T.T., Kroon J., Stroes E.S., Kjaer A., Ochando J., Reiner T., Pérez-Medina C., Calcagno C., Fischer E.A., Zhang B., Temel R.E., Swirski F.K., Nahrendorf M., Fayad Z.A., Lutgens E., Mulder W.J, & Duivenvoorden R. (2018). Efficacy and safety assessment of a TRAF6-targeted nanoimmunotherapy in atherosclerotic mice and non-human primates. Nature biomedical engineering, 2(5), 279-292.
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2

NF-κB Activation Assay in RAW264.7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
RAW264.7 cells, stably transfected with the 3x-κBluc plasmid9 (link), were incubated with the small molecules (10 μM) or vehicle for 1 h. Subsequently, cells were activated using (LPS) from E. coli (Sigma-Aldrich). After 2 h, cells were lysed, and substrate was added according to the manufacturer’s protocol (Luc-screen system; Applied Biosystems). Emission was measured at 450 nm using the Wallac Victor II luminometer.
Lameijer M., Binderup T., van Leent M.M., Senders M.L., Fay F., Malkus J., Sanchez-Gaytan B.L., Teunissen A.J., Karakatsanis N., Robson P., Zhou X., Ye Y., Wojtkiewicz G., Tang J., Seijkens T.T., Kroon J., Stroes E.S., Kjaer A., Ochando J., Reiner T., Pérez-Medina C., Calcagno C., Fischer E.A., Zhang B., Temel R.E., Swirski F.K., Nahrendorf M., Fayad Z.A., Lutgens E., Mulder W.J, & Duivenvoorden R. (2018). Efficacy and safety assessment of a TRAF6-targeted nanoimmunotherapy in atherosclerotic mice and non-human primates. Nature biomedical engineering, 2(5), 279-292.
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