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Anti β actin sc 1615 r

Manufactured by Santa Cruz Biotechnology
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Anti-β-actin (sc-1615-R) is a rabbit polyclonal antibody that specifically recognizes the β-actin protein. β-actin is a highly conserved cytoskeletal protein involved in various cellular processes.

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Lab products found in correlation

Anti fak, by Cell Signaling Technology (1 mentions) Anti p fak, by Abcam (1 mentions) Anti p pten, by Cell Signaling Technology (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti gsk3β, by Cell Signaling Technology (1 mentions) Anti p gsk3β ser9, by Cell Signaling Technology (1 mentions) Anti p akt ser473, by Cell Signaling Technology (1 mentions)

2 protocols using anti β actin sc 1615 r

1

Quantitative Western Blot Analysis

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Western blotting was performed according to standard methods as described previously (13 (link)) using anti-PTEN (cat. no. 9559; Cell Signaling Technology, Inc., Danvers, MA, USA; dilution 1:10,000), anti-p-PTEN (Ser380/Thr382/Thr383; cat. no. 9554; Cell Signaling Technology, Inc.; dilution 1:10,000), anti-FAK (cat. no. ab40794; Abcam; 1:1,000), anti-p-FAK (Tyr397; cat. no. ab4803; Abcam; 1:1,000) and anti-β-actin (sc-1615-R; 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) antibodies. Quantity One software (Bio-Rad Laboratories, Inc., Hercules, CA, USA; version 4.5.1) was used for densitometric measurements of band intensities.
Yang Z., Cao X., Xu W., Xie C., Chen J., Zhu Y, & Lu N. (2017). Phosphorylation of phosphatase and tensin homolog induced by Helicobacter pylori promotes cell invasion by activation of focal adhesion kinase. Oncology Letters, 15(1), 1051-1057.
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2

Western Blotting of Cell Signaling Proteins

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Western blotting was performed according to standard methods, as described previously [16 (link)], using the following antibodies: anti-Akt (#4691; 1 : 1000), anti-p-Akt (Ser473) (#9271; 1 : 1000), anti-GSK-3β (#12456; 1 : 1000), anti-p-GSK-3β (Ser9) (#9323; 1 : 1000) (Cell Signaling Technology, Danvers, MA, USA), anti-APE1 (ab189474; 1 : 1000) (Abcam), and anti-β-actin (sc-1615-R; 1 : 1000; Santa Cruz Biotechnology, Dallas, TX, USA).
Xie C., Yi J., Lu J., Nie M., Huang M., Rong J., Zhu Z., Chen J., Zhou X., Li B., Chen H., Lu N, & Shu X. (2018). N-Acetylcysteine Reduces ROS-Mediated Oxidative DNA Damage and PI3K/Akt Pathway Activation Induced by Helicobacter pylori Infection. Oxidative Medicine and Cellular Longevity, 2018, 1874985.
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