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2 protocols using trimethyl trimesate

1

Doxorubicin-Loaded Nanoscale Metal-Organic Frameworks

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Doxorubicin (DOX, 98%), citric acid (≥99.5%), sodium phosphate monobasic dihydrate (≥99%, NaH2PO4·2H2O), hydrochloric acid (HCl, 34–37%), sodium hydroxide (NaOH, ≥97%) potassium chloride (powder, ≥99%) and deferoxamine mesylate salt (≥92.5%) were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). γ-CD was purchased from Cyclolab (Budapest, Hungary). Dimethylsulfoxide (DMSO, ACS) was from Panreac Applichem (Barcelona, Spain). Deuterated DMSO (DMSO-d6, 99.8% D) and water (D2O, 99.9% D) were obtained from Eurisotop (Gif sur Yvette, France). 1,5-13C2 citric acid was obtained from CortecNet (Les Ulis, France). 1,3,5-benzenetricarboxylic acid (BTC, 95%, Sigma-Aldrich, Saint-Quentin-Fallavier, France), aluminum nitrate nonahydrate (98%, Sigma-Aldrich, Saint-Quentin-Fallavier, France) and trimethyl trimesate (98%, Sigma-Aldrich, Saint-Quentin-Fallavier, France) and absolute ethanol (99%, Carlo Erba, Val-de-Reuil, France) were used for the synthesis of nanoMOFs. Water was purified by a Millipore MilliQ system.
Phosphate buffer saline (PBS, pH 7.4, containing 9.5 mM phosphates) was purchased from Life Technologies (Saint-Aubin, France) and was used for DOX release study.
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2

Synthesis of nanoMOF-based lipid vesicles

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Materials 1,3,5-benzenetricarboxylic acid (trimesate, 95%, Sigma-Aldrich, France) aluminum nitrate nonahydrate (98%, Sigma-Aldrich, France), trimethyl trimesate (98%, Sigma-Aldrich, Saint-Quentin-Fallavier, France) and absolute methanol (99%, Carlo Erba, France) were used for the synthesis of nanoMOFs. FO and FP were purchased from Sigma-Aldrich (France) and Avanti Polar Lipids (France), respectively. Absolute ethanol (99%, Carlo Erba, France) was used for FP@nanoMIL-100. Water was purified by a Millipore MilliQ system.
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