Western blot analysis was performed as previously described (9 (link)) with anti-phospho-IkBα (Ser32) (clone 14D4, Cell Signaling), anti-IkBα (clone L35A5, Cell Signaling), anti-NRF2 (clone D1Z9C, Cell Signaling), and anti-β-actin (clone AC-15, Sigma-Aldrich) Abs. The density of each band was quantified with Imagequant TL (GE Healthcare).
Anti phospho ikbα ser32 clone 14d4
Manufactured by Cell Signaling Technology
Anti-phospho-IkBα (Ser32) (clone 14D4) is a monoclonal antibody that recognizes the Ser32-phosphorylated form of the IkBα protein. IkBα is a regulatory protein that inhibits the NF-kB transcription factor by sequestering it in the cytoplasm. Phosphorylation of IkBα at Ser32 leads to its degradation, allowing NF-kB to translocate to the nucleus and activate target genes.
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2 protocols using anti phospho ikbα ser32 clone 14d4
1
Western Blot Analysis of Signaling Proteins
2
Western Blot Analysis of NRF2 and IkBα
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Western blot analysis was performed as previously described (Kitamura et al. 2012 ) with anti-phospho-IkBα (Ser32) (clone 14D4, Cell Signaling), anti-NRF2 (clone D1Z9C, (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted February 15, 2022. ; https://doi.org/10.1101/2022.02.08.479651 doi: bioRxiv preprint 1 5
Cell Signaling), and anti-β-actin (clone AC-15, Sigma-Aldrich) Abs.
The copyright holder for this preprint this version posted February 15, 2022. ; https://doi.org/10.1101/2022.02.08.479651 doi: bioRxiv preprint 1 5
Cell Signaling), and anti-β-actin (clone AC-15, Sigma-Aldrich) Abs.
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