Goat anti rabbit secondary antibody tagged with texas red t 2767

Sulforaphane (SFN) and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ganetespib (G) and enzalutamide (ENZ) were obtained from ApexBio (Houston, TX, USA). Cycloheximide (CHX) was purchased from Cayman chemicals (Ann Arbor, MI, USA). MG132 was obtained from EMD Millipore (Billerica, MA, USA). Bardoxolone methyl was purchased from Selleckchem (Houston, TX, USA). All drugs were dissolved in 100% DMSO and diluted in media immediately before use. The final DMSO concentration used in experiments was less than 0.1%. The primary antibodies including rabbit polyclonal anti-AR (N-20) (sc-816) and mouse monoclonal anti-GAPDH (sc-47724) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A mouse monoclonal antibody against ubiquitinated proteins (FK2) (BML-PW8810) was obtained from Enzo Life Sciences (Farmingdale, NY, USA). The horseradish peroxidase (HRP)-conjugated goat anti-rabbit (A0545) and goat anti-mouse (A9044) secondary antibodies were purchased from Sigma-Aldrich. The goat anti-rabbit secondary antibody tagged with Texas red (T-2767) was purchased from Thermo Fisher Scientific (Rockford, IL, USA).