100 mm petri dishes

Manufactured by Thermo Fisher Scientific

Sourced in Denmark

The 100 mm Petri dishes are laboratory glassware used for culturing microorganisms, cell lines, or other biological samples. They provide a controlled environment for the growth and observation of these specimens.

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Lab products found in correlation

Cell surface protein isolation kit, by Thermo Fisher Scientific (1 mentions) Anti fas antibody, by Merck Group (1 mentions) Ifn γ, by Hycult Biotech (1 mentions) Neutral red solution, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Absolute digimatic, by Mitutoyo (1 mentions) Sb431542, by Bio-Techne (1 mentions) Plasmocin prophylactic, by InvivoGen (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Pe annexin 5 apoptosis detection kit, by BD (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions)

8 protocols using 100 mm petri dishes

Cell Surface Protein Isolation and Morphology Examination

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Cell surface proteins were biotinylated and isolated by using a Cell Surface Protein Isolation kit (Pierce Biotechnology, Rockford, IL, USA) (20) . After A549 cells were grown to 90-95% confluence in 100 mm Petri dishes (Nunc) and treated with IL-1b, incubation was done for 30 min at 4 C with PBS containing sulphosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate (sulpho-NHS-SS-biotin), a membrane-impermeable biotinylation reagent. Then cells were lysed in the presence of protease inhibitor cocktail, following which biotinylated proteins were isolated using NeutrAvidin agarose. Subsequently, the isolated proteins were released by incubation at room temperature for 60 min with SDS-PAGE sample buffer containing dithiothreitol, followed by immunoblotting.
Examination of cell morphology A549 cells were cultured in 60 mm Petri dishes (Nunc) and were treated with IL-1b and TGF-b1 in the presence of 10% FCS. Phase contrast images were recorded at 30 h using an EVOS f1 (Advanced Microscopy Group, Bothell, WA, USA).

Nakayama I., Higa-Nakamine S., Uehara A., Sugahara K., Kakinohana M, & Yamamoto H. (2020). Regulation of epidermal growth factor receptor expression and morphology of lung epithelial cells by interleukin-1β. Journal of biochemistry, 168(2).

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Modulating HK2 cell response to inflammatory stimuli

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Human kidney (HK2) cells were used. They were grown in RPMI 1640 (Bio Whittaker Labs) supplemented with 10% FCS, 1 mM L-glutamine, 0.66 mg/ml penicillin, 60 mg/ml streptomycin sulfate, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml selenium, in an atmosphere of 95% air/5% CO₂ at 37°C. Cells were seeded in 100-mm Petri dishes (Nunc, Roskilde, Denmark). HK2 cells were treated during 1 and 2 days with TGF-β1 (1 ng/ml; Upstate-Millipore, Madrid, Spain), TNF-α (25 ng/ml; Hycult Biotech, Uden, The Netherlands), IFN-γ (50 ng/ml; Hycult Biotech), stimulatory anti-Fas antibody (0.5 μg/ml; clone CH11, Millipore, Madrid, Spain), and cycloheximide (100 µM).

García-Sánchez O., López-Novoa J.M, & López-Hernández F.J. (2014). Interferon-γ Reduces the Proliferation of Primed Human Renal Tubular Cells. Nephron Extra, 4(1), 1-7.

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Cytotoxicity Assessment of Dental Materials

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To assess the cytotoxicity of the tested paste, an agar overlay assay was performed in accordance with ISO 10993-5:2009. The L929 cells from the Korean Cell Line Bank (Seoul, Korea) were seeded onto 100 mm Petri dishes (Nunc, Roskilde, Denmark) and incubated at 37°C and 5% CO₂ until the concentrations of the cells exceeded 3 × 10⁵/mL. The agar media was created from 3% agar and minimum essential medium-α (HyClone Laboratories, Logan, UT, USA) containing 5% fetal bovine serum (HyClone Laboratories) in the ratio of 1:1. The agar medium was stained with a 0.01% neutral red solution (Sigma-Aldrich, St. Louis, MO, USA) for 2 hours. After removing the stain solution, we placed the Teflon rings with an internal diameter of 10 mm and height of 1 mm on the agar prior to inserting the material. We then inserted each tested material into the ring. A paper disc absorbing the distilled water was considered as a control. Each Petri dish was subsequently incubated at 37°C and 5% CO₂ for 24 hours. The diameter of the decolorized area was measured using digital calipers (Absolute Digimatic, Mitutoyo, Kawasaki, Japan) and recorded. The test was repeated 3 times to ensure reliability.

Lim M.J., Jang H.J., Yu M.K., Lee K.W, & Min K.S. (2017). Removal efficacy and cytotoxicity of a calcium hydroxide paste using N-2-methyl-pyrrolidone as a vehicle. Restorative Dentistry & Endodontics, 42(4), 290-300.

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Tubular Cell Lines Respond to TGF-β1

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Tubular cell lines, namely Human Kidney (HK2) cells and Madin-Darby Canine Kidney (MDCK) cells, were used and grown in an atmosphere of 95% air and 5% CO 2 at 37°C. MDCK were grown in DMEM medium (Bio Whittaker Labs, Rockland ME, USA) supplemented with 10% fetal calf serum (FCS, Bio Whittaker Labs), 0.66 mg/mL penicillin and 60 mg/mL streptomycin sulfate (Bio Whittaker Labs), HK2 were grown in RPMI 1640 (Bio Whittaker Labs) supplemented with 10% FCS, 1 mM L-glutamine, 0.66 mg/mL penicillin, 60 mg/ mL streptomycin sulfate, 5 mg/mL insulin, 5 mg/mL transferrin and 5 ng/mL selenium. Cells were seeded in 100 mm Petri dishes (Nunc, Roskilde, Denmark). MDCK and HK2 cells were treated for 15, 30, 60, 120 minutes and 24, 48, 96 hours with TGF-β1 (0.1, 0.3 and 1 ng/mL) (Upstate-Millipore, Madrid, Spain). In some experiments, the ALK-5 pathway was inhibited with the ALK-5 inhibitor SB431542 (Tocris Bioscience, Bristol, UK). Representative photographs (x200) were obtained of MDCK and HK2 cells treated with 1 ng/ mL TGF-ß1 (or vehicle, as control). Cell proliferation and cell death, and the underlying signaling involved were studied.

García-Sánchez O., Sancho-Martínez S.M., López-Novoa J.M, & López-Hernández F.J. (2015). Activation of the ALK-5 Pathway is not per se Sufficient for the Antiproliferative Effect of TGF-β1 on Renal Tubule Epithelial Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 37(4).

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MCF-7 Cell Aggregate Culture Using Hanging Drop Method

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MCF-7 human breast cancer cells were cultured in 75 cm² T-flasks in DMEM supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin and 0.1% (v/v) Plasmocin Prophylactic (InvivoGen, San Diego, CA, United States) at 37 °C in humidified air with 5% CO₂, and the medium was refreshed every two days. The cell aggregates were cultured using hanging drop method as follows: 1), after detached from the flask, the cells were suspended in culture medium at 4000 cells/mL; 2), drops of 25 μL of the medium with cells were placed on the inner surface of the covers of 100-mm petri dishes (Fisher Scientific); 3), the covers with drops were then inverted and put on the petri dishes containing 8 mL of PBS; and 4), the cells were cultured at 37 °C for 3 days to obtain the MCF-7 cell aggregates. The cell aggregates were then transferred and suspended in 2% (w/v) sodium alginate in saline for further use.

Sun M., Durkin P., Li J., Toth T.L, & He X. (2018). Label-free on-chip selective extraction of cell aggregate-laden microcapsules from oil into aqueous solution with optical sensor and dielectrophoresis. ACS sensors, 3(2), 410-417.

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Antimicrobial Susceptibility Testing Protocol

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PAO-1 cultures were grown overnight to a concentration of 2 × 10⁶. 100 μL of the culture was spread onto 100 mm petri dishes (Fisher Scientific, no. FB0875712) using 30 mm cell spreaders (Fisher Scientific, no. 08-100-10) on Mueller-Hinton agar (BD chemical, ref: 225250). Blank diffusion disks (ThermoFisher, ref: R55054) were soaked in pure LB broth, or LB broth with the desired drug concentrations of L-methionine and ivacaftor for 15 min before being transferred onto the agar with forceps. Plates were incubated at 30 °C overnight and measured the next day.

Cho D.Y., Lim D.J., Mackey C., Weeks C.G., Garcia J.A., Skinner D., Grayson J.W., Hill H.S., Alexander D.K., Zhang S, & Woodworth B.A. (2018). L-methionine Anti-Biofilm Activity against Pseudomonas Aeruginosa Is Enhanced by the CFTR Potentiator, Ivacaftor. International forum of allergy & rhinology, 8(5), 577-583.

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Macrophage Differentiation and Myelin Uptake

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Hematopoietic stem cells were isolated from the femur and tibia bones from WT and TASTPM mice and allowed to differentiate into macrophages for 7 days at 37 °C 95%O₂/5% CO₂ conditions in high glucose Dulbecco's modified Eagle Media (DMEM) supplemented with 10% heat inactivated fetal bovine serum (FBS, Sigma Aldrich), 1% penicillin/streptomycin (P/S, ThermoFisher) and 10% supernatant derived from L929 fibroblast culture as a source of macrophage colony-stimulating factor (Englen et al., 1995 (link)) in 100 mm Petri dishes (Thermo Fisher). On day 5, additional 5 mL of fresh medium was added. On day 7, cells were gently dislodged using a cell scrapper and centrifuged for collection (5 min at 300×g at 4 °C). Cells were plated at 1 × 10⁶/well density in a 12 well-plate and left to set overnight in DMEM supplemented with 1% FBS (Sigma Aldrich) and 1% P/S (ThermoFisher). The next day cells were incubated with 20 μg APC-labelled myelin extracts from WT and TASTPM mice and incubated for 2 h at 37 °C under 95%O₂/5% CO₂ conditions. This experiment was performed 3 independent times, with each replicate of each genotype containing at least 4 animals, unless stated otherwise.

Silva R., Sideris-Lampretsas G., Fox S., Zeboudj L, & Malcangio M. (2022). CD206+/MHCII− macrophage accumulation at nerve injury site correlates with attenuation of allodynia in TASTPM mouse model of Alzheimer's disease. Brain, Behavior, & Immunity - Health, 26, 100548.

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Apoptosis Assay of PAMAM Dendrimer and Lapatinib

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Apoptosis assay was performed using the PE Annexin V apoptosis Detection Kit − 559,763 (BD Biosciences, USA) per manufacturer’s instructions. SKBR3 and ZR75 cells (1 × 10⁶ cells/dish) were seeded in 100 mm petri dishes (Thermo Fisher Scientific, USA) and left to adhere overnight. Cells were treated with PAMAM dendrimers as well as with lapatinib for 48 h. Cell populations were harvested, collected by trypsinization and washed with ice-cold PBS. Then, cells were resuspended in 200 µl of binding buffer. PE Annexin V apoptosis Detection Kit (BD Pharmingen, USA) was used to quantify cell apoptosis in treated versus untreated cells as per the manufacturer’s protocol. Briefly, 5 µl of PE Annexin V-and 5 µl of 7-AAD were added to the samples for 15 min in the dark. Controls were stained with PE Annexin V (no 7-AAD) and 7-AAD (no PE Annexin V). Samples were analyzed by Accuri C6 flow cytometer (BD Biosciences, USA). Data and figures were processed using the FlowJo V10 software and presented as density plots of PE Annexin V and 7-AAD staining.

Kheraldine H., Gupta I., Alhussain H., Jabeen A., Cyprian F.S., Akhtar S., Al Moustafa A.E, & Rachid O. (2021). Substantial cell apoptosis provoked by naked PAMAM dendrimers in HER2-positive human breast cancer via JNK and ERK1/ERK2 signalling pathways. Computational and Structural Biotechnology Journal, 19, 2881-2890.

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