Protein extracts were generated as previously described (18 (link)). Samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen) and then transferred to Immobilon P membranes (Millipore, Temecula, CA). Proteins were detected using rabbit anti-LOXL2 (Abcam, Cambridge, MA) and β-actin antibodies (Santa Cruz Biotechnology) listed in Supplementary Table 2 and enhanced chemiluminescence detection reagents (Amersham, Piscataway, NJ).
Anti loxl2
Manufactured by Abcam
Anti-LOXL2 is a primary antibody that specifically binds to the Lysyl Oxidase-Like 2 (LOXL2) protein. LOXL2 is an enzyme involved in the cross-linking of collagen and elastin in the extracellular matrix. This antibody can be used for the detection and analysis of LOXL2 in various biological samples and applications.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence detection reagent, by Cytiva (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)
Immobilon p membrane, by Merck Group (1 mentions)
Azithromycin, by Merck Group (1 mentions)
Anti vimentin, by Proteintech (1 mentions)
Anti collagen 1, by Proteintech (1 mentions)
Anti alpha smooth muscle actin α sma, by Proteintech (1 mentions)
Anti p jnk, by Proteintech (1 mentions)
Anti α tubulin, by Proteintech (1 mentions)
Anti smad2, by Cell Signaling Technology (1 mentions)
Anti smad3, by Cell Signaling Technology (1 mentions)
Anti c jun, by Proteintech (1 mentions)
Anti p smad3, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Anti jnk, by Proteintech (1 mentions)
Anti tgf β1, by Proteintech (1 mentions)
2 protocols using anti loxl2
1
Western Blot Analysis of LOXL2 Protein
2
Fibrosis Signaling Pathway Modulation
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BLM and the JNK inhibitor (SP600125) were obtained from Selleck China Inc. (Shanghai, China). TGF-β1 was purchased from PeproTech China Inc. (Suzhou, China). Azithromycin was obtained from Sigma-Aldrich Inc. (Shanghai, China). The primary antibodies we used are as follows: anti-vimentin (Proteintech, 60330-1-Ig), anti-alpha-smooth muscle actin (α-SMA) (Proteintech, 14395-1-AP), anti-Collagen 1 (Proteintech, 14695-1-AP), anti-LOX (Proteintech, 17958-1-AP), anti-LOXL2 (Abcam, 96233), anti-TGF-β1 (Proteintech, 21898-1-AP), anti-Smad2 (Cell Signaling Technology, 5339), anti-Smad3 (Cell Signaling Technology, 9523), anti-phospho (P)-smad2 (Cell Signaling Technology, 3108), anti-P-smad3 (Cell Signaling Technology, 9520), anti-JNK (Proteintech, 66210-1-Ig), anti-c-Jun (Proteintech, 66313-1-Ig), anti-P-JNK (Proteintech, 80024-1-RR), anti-P-cJun (Proteintech, 28891-1-AP), anti-α-tubulin (Proteintech, 66031-1-Ig), and anti-GAPDH (Proteintech, 60004-1-Ig). The dilution ratio of all antibodies was 1:1000.
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