Half brains of 22 week old male and female Non-Tg and SUMO1-Tg mice were dissected into 10% buffered formalin and kept at 4 oC overnight. Samples were then embedded in paraffin and sagittal sections were probed with primary antibodies corresponding to rabbit anti-SUMO1 (1:1000), mouse anti-SUMO1 (1:100; MAB 5718 Cell Signaling Technology), mouse anti-Synaptophysin (1:200; 61880 BD Biosciences), rabbit anti-NeuN (1:200; ABN78 Millipore), and mouse anti-GFAP (1:100; MAB 3670 Cell Signaling Technology). Alexafluor 488-conjugated anti-rabbit (1:1000; A20216 Invitrogen) and Cy3-conjugated anti-mouse (1:1000; 115–165-146 Jackson ImmunoResearch) were used as secondary antibodies.
A20216
Manufactured by Thermo Fisher Scientific
The A20216 is a laboratory centrifuge designed for general-purpose applications. It is capable of separating materials by centrifugal force. The centrifuge can accommodate a variety of sample sizes and tube types, and it is equipped with safety features to ensure proper operation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using a20216
1
SUMO1 Localization in Mouse Brains
2
Immunohistochemical Analysis of S6-RP and ID3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standard immunohistology was performed on free-floating sections. Therefore, PFA fixed brains were infiltrated with 30% sucrose in PBS for 48 hours, cut into 40 mm sections, and stored in cryoprotectant (25% Glycerol, 25% Ethylene Glycol, 50% Phosphate Buffer pH 7.8) at À20 C until further use. Tissue sections were incubated with primary antibodies at the following dilutions: rabbit anti-S6-RP (1:50; Cell Signaling #2217S) or rabbit anti-ID3 (1:100; Abcam #Ab41834). Bound primary antibody was visualized using donkey anti-Rabbit antisera conjugated with Alexa 488 (1:500; Invitrogen #A-20216) for S6-RP or the tyramide signal amplification kit (PerkinElmer #NEL701A001KT) for ID3. Pictures were taken on a confocal SPE microscope. We counted individual ID3 positive cells and quantified the mean gray value for S6-RP in the SVZ using the software ImageJ.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!