Five-week-old female inbred C3H/HeN (harboring wild-type TLR4 and simply referred to as C3H), C3H/HeJ (TLR4-deficient), and C57BL/6 mice were purchased from Charles River Japan (Kanagawa, Japan) and acclimated for seven days before the experiment. Mice were housed individually under controlled lighting (with light on from 8 : 00 to 20 : 00 h), temperature (24 ± 0.5°C), humidity (50 ± 10%), and ventilation (~35 complete air exchanges per hour). Food (CRF1; Charles River Japan, Kanagawa, Japan) and water were made available ad libitum.
C3h hej
Manufactured by Charles River Laboratories
Sourced in Japan
The C3H/HeJ is a mouse strain that is a model for studying immune system responses. It is characterized by a genetic deficiency in the Toll-like receptor 4 (TLR4) signaling pathway, which is involved in the recognition of bacterial lipopolysaccharide (LPS). This strain is widely used in biomedical research to investigate the role of TLR4 and the immune system's response to LPS.
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2 protocols using c3h hej
1
Inbred Mouse Model Comparison
2
Mouse Strain Procurement and Housing
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All mice were housed at the laboratory animal facility that had been accredited by AAALAC (Association for Assessment and Accreditation of Laboratory Animal Care International) and the IACUC (Institutional Animal Care and Use Committee) of Tsinghua University. Mouse animal protocols were approved by the laboratory animal facility. C57BL/6, C3H/HEJ, and DBA/2 mice were purchased from Charles River. We obtained B6.SJL-PtprcaPepcb/BoyJ mice from Dr. Li Wu’s Lab (School of Life Science, Tsinghua University).
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