Mir control

Manufactured by Genechem

Sourced in China

The MiR-control is a laboratory equipment used for the analysis and manipulation of microRNAs (miRNAs). Its core function is to provide researchers with a platform for the accurate quantification, detection, and profiling of miRNA expression levels in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Small interfering rna sirna, by GenePharma (1 mentions) Mir control, by GenePharma (1 mentions) Mir 375 mimic, by GenePharma (1 mentions) Puromycin, by Merck Group (1 mentions) Mir 375 mimic, by Genechem (1 mentions)

Spelling variants of this product

MiRNA controls (miR‐NC) (2 citations) MiR-1298-5p overexpression and control lentiviruses (2 citations)

2 protocols using mir control

Transfection of miR-92a and Foxa2 siRNA

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Synthetic miR-92a and the scrambled negative control RNA (miR-control) were purchased from (Genechem, Shanghai, China). All cells were cultured in 6-well plates.
Small interfering RNA (siRNA) sequence targeting human Foxa2 cDNA was synthesized from GenePharma in the RNA interference assay (Shanghai, China). The scrambled siRNA was used as a negative control (NC). When the cells growth reached to 70% confluent, Lipofectamine 2000 (Invitrogen) was used for transfection. Equal amounts of miR-92a or miR-control or relative plasmids were added to each well, 48 hr after transfection, the cells were harvested for qRT-PCR and western blotting.

Wang L., Wu J, & Xie C. (2017). miR-92a promotes hepatocellular carcinoma cells proliferation and invasion by FOXA2 targeting. Iranian Journal of Basic Medical Sciences, 20(7), 783-790.

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Overexpression of miR-375 in Glioma Cells

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MiR-375 mimic and miR-control were purchased from GenePharma (Shanghai, China). MiRNA transfections were performed using Lipofectamine 2000 (Invitrogen, Carlsbad, USA), according to the manufacturer's instructions. The extent of overexpression was evaluated by qRT-PCR 24 h after transfection. MiR-375 mimic and miR-control lentiviruses were synthesised by Genechem (Shanghai, China). For stable overexpression of miR-375 in glioma cells, MiR-375 mimic or miR-control lentiviruses were added to cells, according to the manufacturer's protocol. After 24 h, clones with stable expression were selected by incubating cells for 3 weeks with 5 μg/mL puromycin (Sigma, St Louis, USA) in complete medium containing 10 % FBS. The sequences of miR-control and MiR-375 mimic were 5 -TTCTCCGAACGTGTCACGT-3 and 5 -TTTGTTCGTTCGGCTCGCGTGA-3 , respectively.

Wang J., Liu Y., Li Y., Chen H., Zhang Y., Liu J., Deng S., Zheng Y., Sun X., Wang J., Chen T., Huang M., & Ke Y. (2020). Selective exosome exclusion of miR-375 by glioma cells promotes glioma progression by activating the CTGF-EGFR pathway.

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