Cells labeled with nanoparticles for 4 hours were collected after trypsinization, and re-suspended in PBS at 106 cells/mL. According to the manufacturer’s instructions, fluorescent-labeled Annexin V medium and propidium iodide/7-aminoactinomycin D (FITC/PE Annexin V Apoptosis Detection Kits, BD Biosciences, San Jose, CA, USA) were added to cell suspension, which was kept at room temperature for 15–20 minutes. The percentages of dead cells and cells undergoing apoptosis were determined using a flow cytometer (FACS Calibur, BD Biosciences).
Fitc pe annexin 5 apoptosis detection kits
Manufactured by BD
Sourced in United States
The FITC/PE Annexin V Apoptosis Detection Kits are a set of reagents used to detect and quantify apoptosis in cells. The kits utilize Annexin V, a calcium-dependent phospholipid-binding protein, conjugated with fluorescent dyes such as FITC or PE to identify cells undergoing apoptosis. The kits provide a simple and reliable method for the detection and analysis of apoptotic cells.
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Lab products found in correlation
2 protocols using fitc pe annexin 5 apoptosis detection kits
1
Quantifying Cell Apoptosis and Death
2
Apoptosis Measurement in Human Skin Fibroblasts
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FITC/PE Annexin V Apoptosis Detection Kits (BD Pharmingen TM ) were used to measure HSF apoptosis after transfection (siRNAs) or infection (recombinant adenovirus) [38] [39] [40] . Briefly, the HSFs were treated with medium alone, siRNA specific for Bcl-xL, negative control siRNA, or Lipofectamine RNAiMAX Reagent (Life Technologies) only for 48 h. The HSFs were washed with PBS, resuspended in 100 µl binding buffer and stained with 5 µl FITC-Annexin V and propidium iodide (PI) for 15 min in the dark; then, 300 µl of binding buffer were added. For the recombinant adenovirus, the HSFs were collected after infection for 48 h. Analyses were performed using a flow cytometer (BD FACSAria™ III system; BD Pharmingen) according to the manufacturer's instructions. The HSFs in the FITC/PEpositive fraction were considered apoptotic cells.
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