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Carbamazepine

Manufactured by Tokyo Chemical Industry
Sourced in Japan

Carbamazepine is a chemical compound used as a laboratory reagent. It is a crystalline solid with a melting point of 193-194°C. Carbamazepine is commonly used in various analytical and research applications.

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2 protocols using carbamazepine

1

In Vitro Permeability Assay

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Ascorbic acid, atenolol, dantrolene sodium, gabapentin, hydrocortisone, Ko143, metoprolol tartrate, naproxen, phenytoin sodium, sulpiride, propranolol HCl, type I rat tail collagen solution, lucifer yellow CH dipotassium salt (LY), fluorescein isothiocyanate (FITC)–dextran (4 and 40 kDa), rhodamine 123, and 7.5% sodium bicarbonate solution were purchased from Sigma-Aldrich (St. Louis, MO, USA). Midazolam was purchased from Cerilliant (Austin, TX, USA). Fexofenadine hydrochloride, carbamazepine, donepezil HCl, and cyclosporine A were purchased from Tokyo Chemical Industry (Tokyo, Japan).
Molecular, Cellular, and Development Biology 131 medium (MCDB131 medium), fetal bovine serum (FBS), basic fibroblast growth factor (bFGF), chemically defined lipid concentrate, N-2-hydroxyethylpiperazine–N′-2-ethanesulfonic acid (HEPES), Hanks’ balanced salt solution (HBSS), 0.25% trypsin-EDTA, penicillin-streptomycin, and Insulin-transferrin-selenium-ethanolamine (ITS) were purchased from Gibco (Grand Island, NY, USA). HPLC grade methanol and water were purchased from Fisher Scientific Korea (Seoul, Republic of Korea).
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2

DILI Compound Exposure and LCA Pre-treatment

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DILI drug compounds were dissolved in DMSO to a final concentration of 1% in cell culture medium. Each drug was exposed in three doses for 24 or 72 h with or without LCA 1-h pre-treatment. The following DILI drugs used in this study were categorized into two types: CM-DILI drugs—cyclosporin A (Wako), carbamazepine (Tokyo Chemical Industry), ketoconazole (Sigma-Aldrich), troglitazone (Wako), bosentan (Toronto Research Chemicals), flutamide (LKT Laboratories), diclofenac (Wako), amoxicillin–clavulanate (amoxicillin, Wako; clavulanate, Matrix Scientific) and methapyrilene (Toronto Research Chemicals); and HC-DILI drugs—tacrine (Cayman), tolcapone (Tocris Bioscience) and acetaminophen (Toronto Research Chemicals).
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