Dulbecco's Modi ed Eagle's Medium (DMEM), fetal bovine serum (FBS), and pen/ strep and trypsin EDTA were purchased from Gibco (Carlsbad, CA, USA). RNA extraction kit was purchased from GeneAll biotechnology (Seol, South Korea). The cDNA synthesis kit was purchased from Fermentas (Frickenhausen, Germany). SYBR Green PCR kit was purchased from Ampliqon (Denmark). Metformin and CGA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). ACC, pACC, AMPK, and pAMPK primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse and rabbit secondary antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Polyvinylidene uoride (PVDF), bovine serum albumin (BSA) Phenyl methyl sulfonyl uoride (PMSF), and anti-protease cocktail were purchased from Roche (Mannheim, Germany). ECL reagents were obtained from GE Healthcare Life Sciences (Piscataway, NJ, USA).
Polyvinylidene uoride (PVDF), bovine serum albumin (BSA) Phenyl methyl sulfonyl uoride (PMSF), and anti-protease cocktail were purchased from Roche (Mannheim, Germany). ECL reagents were obtained from GE Healthcare Life Sciences (Piscataway, NJ, USA).