Lysis buffer (#AS1004, Aspen, South Africa) containing 1% protease inhibitor (#AS1008, Aspen) was used to lyse cells or callus samples, after which protein was separated via SDS-PAGE and transferred to NC membranes (#IPVH00010, Millipore, USA) that were blocked with 5% nonfat milk and stained overnight at 4 °C overnight with antibodies specific for collagen I (1:500, Sigma, USA,#ab34710), ALP (1:1000, Sigma, USA,#ab95462), Osteocalcin (1:500, Sigma, USA, #ab93876), RunX2 (1:500, Sigma, USA, #ab23981), SIK2 (1:1000, Sigma, USA, #SAB1302059), SIK3 (1:1000, Sigma, USA, #SAB3500695), and GAPDH (1:10,000, Sigma, USA, #ab37168). Blots were then stained with appropriate secondary antibodies conjugated to horseradish peroxidase (HRP) (#AS1058, Aspen), and proteins were detected with a chemiluminescence detection system. Each experiment was repeated three times.
Sab3500695
Manufactured by Merck Group
Sourced in United States
The SAB3500695 is a laboratory equipment product. It is designed for general laboratory use. The core function of this product is to provide a tool for conducting various scientific experiments and analyses in a laboratory setting. No further details are available about the specific features or intended applications of this product.
Automatically generated - may contain errors
Lab products found in correlation
Ab95462, by Merck Group (3 mentions)
Ab93876, by Merck Group (3 mentions)
Ab23981, by Merck Group (3 mentions)
Ab37168, by Merck Group (3 mentions)
Ab34710, by Merck Group (3 mentions)
As1004, by Aspen (3 mentions)
As1008, by Aspen (3 mentions)
Sab1302059, by Merck Group (3 mentions)
As1058, by Aspen (3 mentions)
Ipvh00010, by Merck Group (3 mentions)
3 protocols using sab3500695
1
Quantitative Protein Analysis of Osteogenic Markers
2
Protein Expression Analysis in Cell/Callus Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysis buffer (#AS1004, Aspen, South Africa) containing 1% protease inhibitor (#AS1008, Aspen) was used to lyse cells or callus samples, after which protein was separated via SDS-PAGE and transferred to NC membranes (#IPVH00010, Millipore, USA) that were blocked with 5% nonfat milk and stained overnight at 4℃ overnight with antibodies speci c for collagen I (1:500, Sigma, USA, #ab34710), ALP (1:1000, Sigma, USA,#ab95462), Osteocalcin (1:500, Sigma, USA, #ab93876), RunX2 (1:500, Sigma, USA, #ab23981), SIK2 (1:1,000, Sigma, USA, #SAB1302059 ), SIK3 (1:1,000, Sigma, USA, #SAB3500695), and GAPDH (1:10,000, Sigma, USA, #ab37168). Blots were then stained with appropriate secondary antibodies conjugated to horseradish peroxidase (HRP) (#AS1058, Aspen), and proteins were detected with a chemiluminescence detection system. Each experiment was repeated three times.
3
Protein Expression Analysis in Cell/Callus Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysis buffer (#AS1004, Aspen, South Africa) containing 1% protease inhibitor (#AS1008, Aspen) was used to lyse cells or callus samples, after which protein was separated via SDS-PAGE and transferred to NC membranes (#IPVH00010, Millipore, USA) that were blocked with 5% nonfat milk and stained overnight at 4℃ overnight with antibodies speci c for collagen I (1:500, Sigma, USA, #ab34710), ALP (1:1000, Sigma, USA,#ab95462), Osteocalcin (1:500, Sigma, USA, #ab93876), RunX2 (1:500, Sigma, USA, #ab23981), SIK2 (1:1,000, Sigma, USA, #SAB1302059 ), SIK3 (1:1,000, Sigma, USA, #SAB3500695), and GAPDH (1:10,000, Sigma, USA, #ab37168). Blots were then stained with appropriate secondary antibodies conjugated to horseradish peroxidase (HRP) (#AS1058, Aspen), and proteins were detected with a chemiluminescence detection system. Each experiment was repeated three times.
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