Stat6 clone sc 621

The biopsy specimen was fixed in buffered formalin and embedded in paraffin for routine histological examination. Immunohistochemical studies were performed on 3-µm sections cut from paraffin blocks using a fully automated system ("Benchmark XT System", Ventana Medical Systems Inc., 1910 Innovation Park Drive, Tucson, Arizona, USA) and the following antibodies: pan cytokeratin (clone AE1/AE3, 1 : 40, Zytomed, Berlin, Germany), vimentin (V9, 1 : 100, Dako, Hamburg, Germany), CK7 (clone OV-TL, 1 : 1000, Biogenex), CK18 (clone CY-90, 1 : 500, Sigma), CK5 (clone XM26, 1 : 50, Zytomed), TTF-1 (clone 8G7G3/1, dilution, 1 : 500, Zytomed), ERG (clone EPR3864, prediluted/ ready to use, Ventana Medical Systems), CD31 (clone JC70A, 1 : 20, Dako), CD10 (clone 56C6, 1 : 20, Dako), p63 (clone SFI-6, 1 : 100, DCS), desmin (clone D33, 1 : 250, Dako), α-smooth muscle actin (clone 1A4, 1 : 200, Dako), S100 protein (polyclonal, 1 : 2500, Dako), CD34 (clone BI-3C5, 1 : 200, Zytomed), CD30 (clone Ber-H2, 1 : 40, Zytomed), MUC4 (clone EP256, 1:500, Epitomics), TLE1 (polyclonal, 1 : 200, Santa Cruz), STAT6 (clone sc-621, 1 : 1000, Santa Cruz Biotechnology), and SMARCB1 (INI1) (MRQ-27, 1 : 50, Zytomed), according to the manufacturer instructions.

All tumour specimens had been fixed in buffered formalin overnight and processed routinely for histological assessment. Immunohistochemistry, on 3 μm sections cut from paraffin blocks, was performed using a fully automated Benchmark XT System (Ventana Medical Systems, Tucson, AR, USA) and the following antibodies: pan-cytokeratin (clone AE1/AE3, 1:40, Zytomed); CK7 (OV-TL, 1:1000, Biogenex); vimentin (V9, 1:100, Dako); desmin (clone D33, 1:250, Dako); α-smooth muscle actin (clone 1A4, 1:200, Dako); h-caldesmon (clone h-CD, 1:100, Dako); protein S-100 (polyclonal, 1:2500, Dako); CD34 (clone BI-3C5, 1:200, Zytomed); ERG (EPR3864, prediluted, Ventana); myogenin (clone F5D, 1:50, Dako); and STAT6 (clone sc-621, 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA).