Urine ALB, TP, NAG, β-2M, and Cr were assayed with the following techniques:
-albumin with the immunoturbidimetric technique, using ROCHE reagents and Cobas Integra 400/800 Analyzer; -TP was assayed with pyrogallol red, using ROCHE reagents and Integra 400/800; -urine NAG was assayed with the colometric technique [15] (link), using Sigma Aldrich (USA) reagents, measuring concentration of the released p-nitrophenyl-2acetamide-2-desoxy-β-2-glucopiranoside; results were read with Perkin Elmer, USA spectrometer; -β-2M was assayed with the chemiluminescence technique using IMMULITE 1000 device (SIEMENS); -Cr was assayed with the Jaffee technique, using ROCHE reagents and Integra 400/800. Training intensity was monitored in both groups using Polar Team-2 Pro, which recorded the heart rate (HR). The procedure consisted of a belt with HR recorder fitted at the chest level, recording HR during the whole training. Then, the HR recorder was detached and put into a device reading changes in HR.
-albumin with the immunoturbidimetric technique, using ROCHE reagents and Cobas Integra 400/800 Analyzer; -TP was assayed with pyrogallol red, using ROCHE reagents and Integra 400/800; -urine NAG was assayed with the colometric technique [15] (link), using Sigma Aldrich (USA) reagents, measuring concentration of the released p-nitrophenyl-2acetamide-2-desoxy-β-2-glucopiranoside; results were read with Perkin Elmer, USA spectrometer; -β-2M was assayed with the chemiluminescence technique using IMMULITE 1000 device (SIEMENS); -Cr was assayed with the Jaffee technique, using ROCHE reagents and Integra 400/800. Training intensity was monitored in both groups using Polar Team-2 Pro, which recorded the heart rate (HR). The procedure consisted of a belt with HR recorder fitted at the chest level, recording HR during the whole training. Then, the HR recorder was detached and put into a device reading changes in HR.