All GC cell lines (BGC823, MGC803, SGC7901, AGS and MKN45) and normal epithelial gastric cells (GES-1) were obtained from Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China). GC cell lines were cultured to 70-80% confluence for subsequent experiments in RPMI-1640 (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and 80 U/ml penicillin at 37°C in humidified air containing 5% CO2. To generate higher transfection efficiency and better results in vitro and in vivo, MGC803 and MKN45 cells were chosen to construct miR-3664-5P overexpression- and knockdown-cell lines, respectively, due to the highest miR-3664-5P expression observed in MKN45 cells and the lowest miR-3664-5P expression observed in MGC803 cells.
Mgc803
Manufactured by Keygen Biotech
Sourced in China
The MGC803 is a laboratory instrument designed for cell culture experiments. It provides precise temperature and environmental control to support the growth and maintenance of cell lines in vitro. The core function of the MGC803 is to create and maintain optimal conditions for cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Ges 1, by Keygen Biotech (5 mentions)
Mkn45, by Keygen Biotech (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Bgc823, by Keygen Biotech (4 mentions)
Hgc27, by Keygen Biotech (4 mentions)
Sgc7901, by Keygen Biotech (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Mkn28, by Keygen Biotech (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Hct116, by Keygen Biotech (1 mentions)
Mcf 7, by Keygen Biotech (1 mentions)
Hek 293, by Keygen Biotech (1 mentions)
Bel 7402, by Keygen Biotech (1 mentions)
Smmc 7721, by Keygen Biotech (1 mentions)
H1299, by Keygen Biotech (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Abcam (1 mentions)
Rpmi 1640 medium, by Abcam (1 mentions)
Dimethyl sulfoxide (dmso), by Cell Signaling Technology (1 mentions)
8 protocols using mgc803
1
Gastric Cancer Cell Line Characterization
2
Cell Line Utilization Protocol
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The human cell lines C4-2B, LNCap (prostate cancer), MGC803, HGC27(gastric carcinoma), SMMC-7721, Bel-7402(hepatic carcinoma), WCY, MCF-7(Breast cancer), H1299, 95D(pulmonary cancer), Hela(cervical cancer), HT29, HCT116(colon carcinoma), LO2(hepatocyte), 2BS(normal lung fibroblast), HEK293(embryonic kidney) and B16(melanoma) origing from mice, were obtained from Nanjing keygen Biotech company. All cell lines were cultured according to the vendor’s instructions.
3
Gastric Cancer Cell Culture Protocol
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Gastric cancer cell lines (AGS and MGC80-3) were purchased from Nanjing KeyGen Biotech Co., Ltd. RPMI 1640 medium (Abcam) containing 10% FBS (Abcam) and 100 U/ml penicillin and 100 µg/ml streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.) was used to cultivate gastric cancer cell lines at 37°C with 5% CO2.
4
Inhibiting PI3K in Gastric Cancer
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Human gastric cancer cell MGC-803 and the normal gastric epithelial cell line GES-1 were purchased from Nanjing KeyGen Biotech Co., Ltd. The cells were cultured in RPMI-1640 medium with 10% FBS (both purchased from Thermo Fisher Scientific, Inc.), 100 µg/ml penicillin and 100 µg/ml streptomycin at 37°C in a humidified incubator at 5% CO2. The PI3K inhibitor LY294002 (10 mmol/l; Cell Signaling Technology, Inc.) was dissolved in DMSO (cat. no. D2650; Sigma-Aldrich; Merck KGaA) and diluted with RPMI-1640 medium to 12.5, 25 and 50 µmol/l. LY294002 was cultured with MGC-803 cells at 37°C for 24 h in a humidified incubator at 5% CO2.
5
Gastric Cancer Cell Line Cultivation
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Seven human GC cell lines (MKN45, MKN28, MGC803, MGC823, SGC7901, HGC27, and BGC823) and normal human gastric epithelial cell lines (GES‐1) were purchased from KeyGEN BioTECH (Nanjing, China). Cells were cultured in at 37°C in a 5% CO2 humidified atmosphere in RPMI‐1640 containing 10% FBS. Recombinant human TGF‐β1 was purchased from Novoprotein. The sequences for Trop2 overexpression vector and control OE vector, Trop2 shRNA and control shRNA vector are listed in Table S1 .
6
Gastric Cancer Cell Line Maintenance
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The human gastric cancer cell lines MGC803, BGC823, MKN45, MKN28, and HGC27 and the gastric epithelial cell line GES-1 were purchased from KeyGEN Biotechnology. Cell lines were maintained in RPMI 1640 medium (#61870044, Gibco, USA) containing 10% fetal bovine serum (#10099141, Invitrogen, USA), 100 units/mL penicillin, and 100 μg/ml streptomycin sulfate (#15070063, Invitrogen, USA). All cells were incubated in a humidified atmosphere containing 5% CO2 at 37°C.
7
Culturing Human Gastric Cell Lines
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The human gastric epithelial cell line GES-1 and GC cell lines AGS, HGC27, MGC803, and MKN45 were purchased from KeyGEN BioTECH (Nanjing, China). GES-1, AGS, and HGC27 cells were cultured in DMEM (Gibco, Waltham, MA, USA), while MGC803 and MKN45 cells were cultured in RPMI-1640 (Gibco). All media were supplemented with 10% fetal bovine serum (FBS, Biological Industries, Beit-Haemek, Israel), 100 KU/L penicillin, and 100 mg/L streptomycin. Cells were maintained in an incubator at 37 °C with 5% CO2.
8
Culturing Human Gastric Cancer Cell Lines
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The AGS, BGC823, MKN45, SGC7901, and MGC803 human GC cell lines were obtained from KeyGEN BioTECH (Nanjing, China). Cells were cultured in RPMI 1640 medium with 10% fetal bovine serum (Thermo Scientific, USA) at 37°C under 5% CO2.
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