1 2 dipalmitoyl sn glycero 3 phosphocho line dppc 1 2 distearoyl sn glycero 3 phosphoethanola mine n amino polyethyleneglycol 2000 dspe peg2000 amine

Lipids, including 1,2-dipalmitoyl-sn-glycero-3-phosphocho-line (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanola-mine-N-[amino(polyethyleneglycol)-2000] (DSPE-PEG2000-Amine), and DC-cholesterol hydrochloride (DC-CHOL) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Chloroform (CHCl₃) was purchased from Chongqing East Chemical Industry Ltd, Co. (Chongqing, China). Superparamagnetic iron oxide nanoparticles (Fe₃O₄, 10 nm, 25 mg/mL) were purchased from Ocean Nanotech Co. Ltd. (San Diego, USA). Perfluorohexanes (PFH, a PFC compound with a boiling point of 56°C) were purchased from Biofroxx Ltd, Co. (Shanghai, China). 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) was purchased from AAT Bioquest Inc. (USA). 2-(4-Amidinophenyl)- 6-indolecarbamidinedihyrochloride(DAPI), 1,1-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) and fluorescein isothiocyanate (FITC) were purchased from Beyotime Biotechnology Co., Ltd (Shanghai, China).

Lipids including 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1, 2-distearoyl-sn-glycero -3-phosphoethanolamine-N-[amino(polyethyleneglycol)-2000] (DSPE-PEG 2000-Amine) and DC-cholesterol hydrochloride (DC-CHOL) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Chloroform (CHCL₃) was purchased from Chongqing East Chemical Industry Ltd, Co. (Chongqing, China). Indocyanine Green (ICG) was obtained from Aladdin (Shanghai, China). 2-(4-Amidinophenyl)-6-indolecarbamidinedihyrochloride (DAPI), 1.1ʹdioctadecy-3,3,3ʹ-tetramethylindocarbocyaninepercholrate (DiI), fluorescein isothiocyanate (FITC) was purchased from Beyotime Biotech Ltd, Co. Perfluorohexanes (PFH, a PFC compound with a boiling point of 56°C) were purchased from Biofroxx Ltd, Co. (Shanghai, China).
All chemicals were analytical grade and used without further purification.