The human breast cancer cell lines MDA-MB-231 and MCF-7, as well as the murine breast cancer cell line 4T1, were obtained from the American Type Culture Collection (USA). The human fibroblast cell line HFCH8 was a kind gift from Dr. Myeong-Sok Lee (Sookmyung Women's University, Korea). MDA-MB-231, MCF-7, and HFCH8 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone Laboratories, USA) at 37°C in a humidified 5% CO 2 incubator as described previously [19] . The 4T1 cells were maintained in RPMI 1640 supplemented with 10% heat-inactivated FBS.
Mcf 7
Manufactured by Cytiva
Sourced in United States
The MCF-7 cell line is a well-established breast cancer cell line that is commonly used in biomedical research. It is a widely-studied model for investigating various aspects of breast cancer biology and drug development. The core function of the MCF-7 cell line is to provide a reliable and standardized in vitro system for researchers to conduct their experiments.
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Lab products found in correlation
Mcf 7, by American Type Culture Collection (6 mentions)
Fetal bovine serum (fbs), by Cytiva (5 mentions)
Glutamax, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Cytiva (3 mentions)
Rpmi 1640, by Cytiva (3 mentions)
Mda mb 231, by Cytiva (2 mentions)
Rpmi 1640 medium, by Cytiva (2 mentions)
Penicillin, by Cytiva (2 mentions)
Skbr3, by Cytiva (2 mentions)
Dcc fbs, by Thermo Fisher Scientific (2 mentions)
Universal mycoplasma detection kit, by American Type Culture Collection (2 mentions)
Dcc fbs, by Cytiva (2 mentions)
Hcc 1428, by Cytiva (2 mentions)
Ethylene diamine tetraacetic acid (edta), by Corning (2 mentions)
Hcc1428, by American Type Culture Collection (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
Skbr3, by American Type Culture Collection (2 mentions)
Hff 1 cells, by American Type Culture Collection (1 mentions)
L glutamine, by GE Healthcare (1 mentions)
Streptomycin, by Cytiva (1 mentions)
10 protocols using mcf 7
1
Breast Cancer Cell Line Culturing
2
Culturing Human Breast Cancer Cell Lines
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Human breast cancer cell lines SK-BR-3 and MCF-7 were purchased from the ATCC (Manassas, VA). SK-BR-3 cells and MCF-7 cells were maintained in Dulbecco's modified Eagle's medium (DMEM; HyClone Laboratories, Logan, UT) and RPMI 1640 medium (HyClone Laboratories) both supplemented with 10% fetal bovine serum (FBS) and 1% penicillin (HyClone Laboratories). Cells were incubated in the cell culture incubator with 5% CO2 at 37°C.
3
Cultivation of Human Cell Lines
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A375 (human malignant melanoma) and MCF7 (human breast adenocarcinoma) cell lines were purchased from ECACC, Salisbury, UK. HFF-1 cells (human foreskin fibroblasts) were obtained from ATCC. A375 and MCF7 cells were cultivated in RPMI 1640 and HFF cells in DMEM medium (HyClone Laboratories, Inc., Logan, UT, USA). Growth media were supplemented with 2 mM L-glutamine (PAA Laboratories, Pasching, Austria), 10% fetal calf serum, 100 IU/ml penicillin, and 100 µg/ml streptomycin (HyClone Laboratories, Inc., Logan, UT, USA). Cells were incubated at 37 °C in a high-humidity atmosphere with 5% CO2 and were passaged three times a week.
4
Culturing Human Cancer Cell Lines
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Human cancer cell lines used in this study (KB, H226B, A549, LNCaP, DU145, SK-BR-3, MCF7, DLD-1, HCT116, A172, and HepG2) were purchased from American Type Culture Collection (Manassas, VA, USA). The multidrug-resistant cell line KBV20C was derived from KB21 (link),22 (link). KB, KBV20C, H226B, A549, LNCaP, DU145, and DLD-1 cells were grown in RPMI-1640 (HyClone Laboratories, Logan, UT, USA), while SK-BR-3, MCF7, HCT116, A172, and HepG2 cells were grown in DMEM (HyClone Laboratories), both supplemented with 10% FBS (HyClone Laboratories) and 100 units/mL of penicillin/streptomycin (HyClone Laboratories). KBV20C cells were maintained with 20 nM vincristine in their cell growth medium. Glioblastoma multiform, U87 cells, a kind gift from Dr. Hyunggee Kim (Korea University), were cultured in DMEM/F12 medium (Invitrogen, Waltham, MA). All cells were maintained at 37 °C under 5% CO2 in a humidified cell culture incubator.
5
3D Culture of Breast Cancer Cell Lines
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All human cell lines were purchased from the American Type Culture Collection (ATCC, USA). MCF-7 (luminal A) and MDA-MB-231 (triple-negative breast cancer (TNBC); claudin-low) were cultured in DMEM, whereas MDA-MB-468 (TNBC; basal) were cultured in RPMI 1640 supplemented with 10% (v/v) thermally inactivated fetal bovine serum (Hyclone Laboratories, USA). To generate a 3D model, cells were seeded in ultra-low attachment round-bottom 96-well plates (Corning, USA) and allowed to form multicellular aggregates after 48 h [16 (link), 17 (link)]. All cells were incubated at 37°C and 5% CO2.
6
Culturing Human Breast Cell Lines
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Human breast cancer cell lines MCF-7, MDA-MB-231, BT474 and normal human breast epithelial cell lines HMLE and MCF-10A were purchased from the Shanghai Institute for Biological Sciences (SIBS), Chinese Academy of Sciences (Shanghai, China). MCF-7, MDA-MB-231 and BT474 were cultured in RPMI 1640 Medium (Hyclone Laboratories, Inc., Logan, UT, USA) and HMLE cells were cultured in DMEM/F-12 1:1 (Hyclone Laboratories, Inc.) containing 10% fetal bovine serum supplemented with 100 units/mL of penicillin and 100 μg/mL of streptomycin. MCF-10A cells were cultured in DMEM/F-12 1:1 containing insulin, hydrocortisone, EGF and 10% horse serum supplemented with 100 units/mL of penicillin and 100 μg/mL of streptomycin. All cell lines were cultured in a humidified incubator at 37 °C with 5% CO2.
7
Breast Cancer Cell Culture Conditions
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Cultures were maintained in standard incubation conditions, 37°C and 5% CO2 culturing media as follows: HMEC (Invitrogen Life Technologies Corporation, Carlsbad, CA, USA) and HuMEC Basal Serum Free Medium (Invitrogen) supplemented with HuMEC Supplement and Bovine Pituitary Extract (Invitrogen); MCF10a (ATCC, Manassas, VA, USA) and Dulbecco's modified Eagle's medium (DMEM)-F12 (Invitrogen) and 5% HS (HyClone Laboratories, UT, US); MCF7 (ATCC) and RPMI1640 (Invitrogen) + 10% FBS (HyClone Laboratories); MDA-MB-231 (ATCC, 2007–2010), DMEM-F12 (Invitrogen) and 10% FBS (HyClone Laboratories); MCF7/DOX: detailed description of culturing and cloning procedures can be found in a previous publication [22 (link)].
8
Establishment of LTED Breast Cancer Cell Lines
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Parental cancer cell lines (MCF-7, HCC-1428, T47D) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA), and LentiX cells were obtained from Clontech (Mountain View, CA, USA). MCF-7, HCC-1428, T47D, and LentiX cells were cultured in DMEM with 10% FBS (HyClone Laboratories; Logan, UT, USA). MCF-7/LTED cells were a gift from Matthew Ellis (Baylor College of Medicine, Houston, TX, USA) (45 (link)). HCC-1428/LTED cells were generated through long-term (>1 yr) culture in phenol-red-free DMEM with 10% dextran/charcoal-treated FBS (DCC-FBS; HyClone Laboratories) as previously described (46 (link)). LTED cells were maintained in phenol-red-free DMEM + 10% DCC-FBS supplemented with 2 mM GlutaMAX (ThermoFisher Scientific, Waltham, MA, USA), and were passaged using phenol-red-free 0.25% trypsin plus 2.21 mM EDTA (Corning, Tewksbury, MA, USA). Cell lines were verified by STR genotyping at the University of Vermont Cancer Center DNA Analysis Facility and confirmed to be free of mycoplasma (Universal Mycoplasma Detection Kit; ATCC). Assays were performed using cells cultured for <3 months thereafter.
9
Cytotoxicity Evaluation of DOX-loaded NPs on MCF-7 Cells
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The cells used in this study were MCF-7 human breast cancer cells purchased from ATCC (American Type Culture Collection; code no ATCC® HTB-22TM). MCF-7 cells were cultured in DMEM (Dulbecco’s Modified Eagle Medium) culture medium (HyClone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (HyClone Laboratories) and 1% of penicillin/streptomycin (Welgene, Daegu, Korea). MCF-7 cells were seeded onto 96-well plates at a density of 5.0 × 103 cells/well and incubated at 37 °C for 24 h. The WST-1 assay was then performed according to the manufacturer’s instructions 48 h after treatment with NPs of DOX-loaded NPs at the indicated concentrations. The absorbance was measured by VICTOR X3 multi-label plate reader (PerkinElmer, Waltham, MA, USA) at 450 nm.
10
Establishment of LTED Breast Cancer Cell Lines
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