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Mrc 5 lung fibroblasts

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MRC-5 lung fibroblasts are a well-characterized cell line derived from normal human fetal lung tissue. They are a commonly used in vitro model for the study of various cellular processes and are suitable for a wide range of applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Human il 6, by R&D Systems (1 mentions) Hek blue il 1β cells, by InvivoGen (1 mentions) Hkb il1b, by InvivoGen (1 mentions) Human il 1β, by Thermo Fisher Scientific (1 mentions) Quanti blue assay, by InvivoGen (1 mentions) Human il 1ra, by Thermo Fisher Scientific (1 mentions) G csf, by R&D Systems (1 mentions) Il 1ra, by InvivoGen (1 mentions) Human il 1α, by Thermo Fisher Scientific (1 mentions) Sl sl stromal cells, by STEMCELL (1 mentions) M2 10b4 stromal cells, by STEMCELL (1 mentions) Hek293t 17 cells, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Cytiva (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Tgf β1, by Merck Group (1 mentions) Mcf 7 breast cancer, by American Type Culture Collection (1 mentions) Hct116 colorectal carcinoma, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

MRC-5 human lung fibroblasts MRC-5 fibroblasts MRC-5

7 protocols using mrc 5 lung fibroblasts

1

Fibroblast Stimulation with TGF-β1 and BLM

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Human MRC5 lung fibroblasts (ATCC, Manassas, VA, USA) were cultured in DMEM supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 100 U/mL penicillin, and 100 µg/mL streptomycin (Gibco). The cells were maintained in a humidified atmosphere with 5% (v/v) CO2 at 37 °C. The cells were stimulated for 48 h in serum-free medium supplemented with 0.1% bovine serum albumin (BSA) and 5 ng/mL TGF-β1 or 10 µg/mL BLM.
Lee J.U., Song K.S., Hong J., Shin H., Park E., Baek J., Park S., Baek A.R., Lee J., Jang A.S., Kim D.J., Chin S.S., Kim U.J., Jeong S.H, & Park S.W. (2024). Role of lung ornithine aminotransferase in idiopathic pulmonary fibrosis: regulation of mitochondrial ROS generation and TGF-β1 activity. Experimental & Molecular Medicine, 56(2), 478-490.
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2

Evaluating IL-1RA and Antibody Therapy in Cell-Based Assays

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40pg/uL human IL-1RA (PeproTech, US) was pre-incubated with 5ng/uL antibody (G4–21 or isotype control. IL-1RA and antibody were applied to HEK-Blue IL-1β cells (InvivoGen, US) for 2 hours and stimulated with 2pg/uL human IL-1β (PeproTech, US) for 36 hours at 37°C. Alternatively, cells were treated with 8–10 nM IL-1RA and commercial monoclonal (mAbs) or polyclonal antibodies (pAbs) at 40–2000nM, or with patient plasma at 1:10 and 1:20 dilutions, and subsequently stimulated with 0.1–1 nM IL-1β for 24 hours. Cells were treated with antibodies in the presence of IL-1α and IL-1RA, IL-1RA alone, or media, and supernatants assayed using the QUANTI-Blue assay (InvivoGen, hkb-il1b, US).
Human A549 lung epithelial cells and MRC-5 lung fibroblasts (ATCC, US) were treated with 10nM IL-1RA and patient plasma and stimulated with 0.5nM human IL-1α (PeproTech, US) for 24 hours at 37°C. RNA was isolated, cDNA prepared, and samples analyzed using TaqMan FAM-conjugated primer sets. Supernatants were collected and assayed for human IL-6 (R&D, US), IL-8 (R&D, US), and G-CSF (R&D, US) via ELISA.
Jarrell J.A., Baker M.C., Perugino C.A., Liu H., Bloom M.S., Maehara T., Wong H.H., Lanz T., Adamska J.Z., Kongpachith S., Sokolove J., Stone J.H., Pillai S.S, & Robinson W.H. (2021). Neutralizing Anti-Interleukin-1 Receptor-Antagonist Autoantibodies Induce Inflammatory and Fibrotic Mediators in IgG4-Related Disease. The Journal of allergy and clinical immunology, 149(1), 358-368.
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3

Latency Assay of CD34+ Hematopoietic Progenitor Cells

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MRC-5 lung fibroblasts (ATCC), HEK293T/17 cells (ATCC), Sl/Sl stromal cells (Stem Cell Technology), M2-10B4 stromal cells (Stem Cell Technology), and CD34+ HPCs were maintained as previously described [17 (link)]. Human CD34+ HPCs were isolated from de-identified medical waste following bone marrow isolations from healthy donors for clinical procedures at the Banner-University Medical Center at the University of Arizona. Latency assays were performed as previously described [15 (link), 17 (link)].
Buehler J., Carpenter E., Zeltzer S., Igarashi S., Rak M., Mikell I., Nelson J.A, & Goodrum F. (2019). Host signaling and EGR1 transcriptional control of human cytomegalovirus replication and latency. PLoS Pathogens, 15(11), e1008037.
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4

Culturing MRC-5 Human Lung Fibroblasts

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MRC-5 lung fibroblasts (human lung fibroblasts; American Type Culture Collection, Manassas, VA, USA; cat. no. CCL 171) were cultured in high Dulbecco’s modified Eagle’s medium (DMEM; HyClone Laboratories, Inc., Logan, UT, USA) with 10% fetal bovine serum (FBS; Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 1% L-glutamine and 1% penicillin/streptomycin solution. Cells were incubated at 37°C in 5% CO2 and routinely passaged upon reaching 80% confluency, using 0.25% trypsin and a 1:3 cell dilution for each passage.
YANG Z., SUN Z., LIU H., REN Y., SHAO D., ZHANG W., LIN J., WOLFRAM J., WANG F, & NIE S. (2015). Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat-induced pulmonary fibrosis. Molecular Medicine Reports, 12(1), 1091-1097.
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5

Cytotoxicity Evaluation of Derrone

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Human MRC-5 lung fibroblasts (American Type Culture Collection, Manassas, VA, USA; ATCC-171) were maintained in DMEM supplemented with 10% FBS and an antibiotic mixture. The cells were maintained at 37 °C in a humidified incubator with 5% CO2. Cell viability was determined using an MTT-based in vitro assay. Briefly, MRC-5 cells were seeded in 24-well plates at a density of 2 × 104 cells/well for 16 h and treated with the indicated concentrations of derrone for 24, 48, and 72 h. Following incubation, MTT was added to each well, and the plates were incubated at 37 °C for 4 h. The content of each well was eluted, and the precipitate was dissolved in dimethyl sulfoxide. Absorbance was measured at 540 nm using a microplate reader (BioTek Instruments Inc., Winooski, VT, USA). Relative cell viability (%) was calculated as the ratio of surviving cells to untreated cells.
Molagoda I.M., Sanjaya S.S., Lee K.T., Choi Y.H., Lee J.H., Lee M.H., Kang C.H., Lee C.M, & Kim G.Y. (2023). Derrone Targeting the TGF Type 1 Receptor Kinase Improves Bleomycin-Mediated Pulmonary Fibrosis through Inhibition of Smad Signaling Pathway. International Journal of Molecular Sciences, 24(8), 7265.
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6

Modulation of TGF-β1-induced Fibroblast Activation

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MRC5 lung fibroblasts (human fetal lung fibroblasts; American Type Culture Collection, Manassas, VA, USA) were grown in Dulbecco's modified Eagle medium (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) with 4.5 g/l glucose, 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% penicillin/streptomycin solution. Cells were maintained at 37°C in a humidified incubator, in the presence of 5% CO2. Following incubation for 3 days, the cells were cultured to ~80% confluence.
MRC5 cells were pretreated with MaR 1 (Cayman Chemical Company, Ann Arbor, MI, USA) at 1, 10 or 100 nM, or vehicle (0.035% ethanol) for 30 min. Subsequently, TGF-β1 (10 ng/ml; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) was added and co-incubated for 24 h.
Sun Q., Wu Y., Zhao F, & Wang J. (2017). Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts. Molecular Medicine Reports, 16(2), 1523-1529.
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7

Culturing Human Cancer Cell Lines

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Human cell lines (A375 melanoma, MCF-7 breast cancer, HCT116 colorectal carcinoma, A2780 ovarian cancer, and MRC5 lung fibroblasts) were purchased from American Type Culture Collection (Rockville, MD, USA).
The A2780 cells were cultivated in DMEM, while all of the other cell lines were cultivated in the HEPES-buffered RPMI-1640 medium. The cell culture mediums were supplemented with penicillin (100 units/mL), streptomycin (100 μg/mL), and 10% inactivated FBS. All of the cells were cultivated at 37 °C in a humidified atmosphere with 5% CO2.
Jevtovic V., Alshamari A.K., Milenković D., Dimitrić Marković J., Marković Z, & Dimić D. (2023). The Effect of Metal Ions (Fe, Co, Ni, and Cu) on the Molecular-Structural, Protein Binding, and Cytotoxic Properties of Metal Pyridoxal-Thiosemicarbazone Complexes. International Journal of Molecular Sciences, 24(15), 11910.
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