N methylpyrrolidinone

All the reagents were used as received. The photoresists: SU-8 5 and S1808 as well as the photoresist developers were purchased from MicroChem. Cytop and the Cytop solvent were obtained from AGC. N-methylpyrrolidinone and ethylene glycol (EG) were purchased from Fischer Scientific. 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (sulfo-NHS) and sulfo-NHS-biotin were purchased from Thermo Scientific. Acetone was obtained from VWR, ethanol from Gold Shield, hydrochloric acid from J. T. Baker and nitric acid from Macron Chemicals. PBS 10 × was obtained from Affymetrix and diluted by 10 with mQ water prior to utilization. Hellmanex, tetraethyl orthosilicate (TEOS), sodium nitrate, (3-aminopropyl)trimethoxysilane (APTMS) and poly(L-glutamic acid) (PGlu) (MW = 50 000-100 000) were purchased from Sigma Aldrich. The aqueous solutions were prepared using milliQ water. The gold nanoparticles (AuNPs) were synthesized using the well-known Turkevich method. The amino-terminated ssDNA sequences were obtained from IDT. Alexa647-streptavidin was purchased from Molecular Probes and Human α-thrombin was obtained from Haematologic Technologies. The glass slides used for the deposition of Cytop were obtained from Fischer. The nickel pellets used for the thermal evaporation were purchased from Kurt J. Lesker.