FACS isolated cells were lysed in Trizol (Ambion). RNA integrity was evaluated using a Eukaryotic RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer (Agilent Technologies). Up to 1 μg of total RNA from each sample was used for library preparation with the TruSeq RNA Sample Preparation Kit (Illumina). A common adaptor was used for all samples and barcode sequences present in the reverse primer were introduced by 12–20 cycles of amplification as described (Pereira et al., 2013 (link)). Each library was assessed for quality and size distribution using an Agilent High Sensitivity Assay bioanalyzer chip and quantified by real-time PCR. Equimolar amounts of each barcoded library were mixed and single-end sequenced on an Illumina HiSeq Sequencing System.
Agilent high sensitivity assay bioanalyzer chip
Manufactured by Agilent Technologies
The Agilent High Sensitivity Assay bioanalyzer chip is a laboratory instrument used for the analysis and quantification of DNA, RNA, and protein samples. It provides sensitive and accurate measurements of sample size, concentration, and purity through the use of microfluidic technology.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using agilent high sensitivity assay bioanalyzer chip
1
RNA-seq Library Preparation from FACS Cells
2
RNA-seq Library Preparation from FACS Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!