The inhibitor of human miR-21 (miR21-In, 107 units/mL), and its non-specific miRNA lentivirus (miR-C, 107 units/mL) were obtained from RiboBio (China). KYN-2 cells were incubated with lentiviral particles (10 mL/1,000 cells, multiplicity of infection=10–15) and polybrene (8 mg/mL) for 48 hours. Thereafter, the media was changed, and liver cancer KYN-2 cells were kept for another 3–7 days for stabile transduction. The cells were then passaged and preserved in vitro for further analysis.
Mir c
Manufactured by RiboBio
Sourced in China
The MiR-C is a laboratory instrument designed for the detection and analysis of microRNA (miRNA) expression. It utilizes advanced technologies to provide accurate and reliable results for researchers studying gene regulation and expression patterns.
Automatically generated - may contain errors
2 protocols using mir c
1
Stable Transduction of Liver Cancer Cells
2
Overexpression of XIAP in BCSCs
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MiR-489 mimics (5′-GUGACAUCACAUAUACGGCAGC-3′) and random RNA oligonucleotides (miR-C, 5′-ACUGAAUACUGCGCAAACGUGC-3′) were purchased from RiboBio Co. Ltd. (Guangzhou, China).XIAP open reading frame was amplified and then linked to the pcDNA3.1 eukaryotic expression vector (Invitrogen) to overexpress the gene of XIAP in BCSCs. For transfection,cells were cultured in 6-well plates overnight followed by transfecting with miR-489 mimics (50 pmol/ml), miR-C (50 pmol/ml) and XIAP plasmid (2 μg/ml) by using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocols. After transfection, cells were collected for the following experiments.
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