Free-floating sections were incubated with a rabbit anti-Crh antibody (diluted 1:5000; a generous gift from the late Dr. Wylie Vale’s lab) overnight at 4°C and Crh IHC staining was performed as described previously.37 (link) Sections were rinsed five times for 5 min in 50mMKPBS and subsequently incubated in biotinylated goat anti-rabbit secondary antibody (diluted 1:500; Vector Laboratories) for 1 h. Following five, 5 min rinses in KPBS, sections were incubated in avidin-biotin complex (diluted 1:1000; Vector Laboratories) for 1 additional hour. Sections were rinsed five times for 5 min in KPBS, incubated in biotin-labeled tyramide (diluted 1:250; PerkinElmer Life Sciences), rinsed five times for 5 min, and incubated in Cy3-conjugated streptavidin (diluted 1:500; Jackson ImmunoResearch) for 30 min. All sections in the series were then examined by fluorescence microscopy to identify positively labeled cells and fibers. Images of the PVH were captured from low to high magnification by using a Nikon E800 microscope and Nikon Elements software.
Biotin labeled tyramide
Manufactured by PerkinElmer
Biotin-labeled tyramide is a reagent used in immunohistochemistry and in situ hybridization techniques. It functions as a signal amplification system to enhance the detection of target molecules. The biotin label allows for subsequent detection and visualization of the labeled target.
Automatically generated - may contain errors
2 protocols using biotin labeled tyramide
1
Crh Immunohistochemistry in Rodent Brain
2
Crh Immunohistochemistry in Rodent Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Free-floating sections were incubated with a rabbit anti-Crh antibody (diluted 1:5000; a generous gift from the late Dr. Wylie Vale’s lab) overnight at 4°C and Crh IHC staining was performed as described previously.37 (link) Sections were rinsed five times for 5 min in 50mMKPBS and subsequently incubated in biotinylated goat anti-rabbit secondary antibody (diluted 1:500; Vector Laboratories) for 1 h. Following five, 5 min rinses in KPBS, sections were incubated in avidin-biotin complex (diluted 1:1000; Vector Laboratories) for 1 additional hour. Sections were rinsed five times for 5 min in KPBS, incubated in biotin-labeled tyramide (diluted 1:250; PerkinElmer Life Sciences), rinsed five times for 5 min, and incubated in Cy3-conjugated streptavidin (diluted 1:500; Jackson ImmunoResearch) for 30 min. All sections in the series were then examined by fluorescence microscopy to identify positively labeled cells and fibers. Images of the PVH were captured from low to high magnification by using a Nikon E800 microscope and Nikon Elements software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!