C57bl 6j wild type and ldl receptor ldlr ko b6.129s7 ldlrtm1her j mice

Human dermal microvascular endothelial cells (hDMVECs) and endothelial cell (EC) culture medium were purchased from ScienCell (San Diego, CA, USA). Phoenix cells and Dulbecco's Modified Eagle Medium (DMEM) were purchased from ATCC (Manassas, VA, USA). Lentiviral vector pLV-mCherry were obtained from Addgene (Watertown, MA, USA). Coding sequence of SARS-CoV-2 Spike gene (Wuhan variant, GenBank: QHU36824.1) and the expression vector were described previously [22 (link)]. LDL-c, HDL-c and lipoprotein depleted fetal bovine serum (LD-FBS) were obtained from Kalen Biomedical, LLC (Germantown, MD, USA). Sulfo-NHS-LC-biotin and desalting spin column were obtained from ThermoFisher (Waltham, MA, USA). The sources of antibodies were listed in the supplementary table 1. Primers were synthesized by IDT (Coralville, IA, USA) and listed in supplementary table 2. The animal protocol was approved by the IACUC committee at the University of South Carolina, Columbia. C57BL/6J wild type and LDL receptor (LDLR) KO (B6.129S7-Ldlr^tm1her/J) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). The generation of Spp virus was previously reported [22 (link)].

Human dermal microvascular endothelial cells (hDMVECs) and endothelial cell (EC) culture medium were purchased from ScienCell (San Diego, CA, USA). Phoenix cells and Dulbecco's Modified Eagle Medium (DMEM) were purchased from ATCC (Manassas, VA, USA). Lentiviral vector pLV-mCherry were obtained from Addgene (Watertown, MA, USA). Coding sequence of SARS-CoV-2 Spike gene (Wuhan variant, GenBank: QHU36824.1) and the expression vector were described previously [22 (link)]. LDL-c, HDL-c and lipoprotein depleted fetal bovine serum (LD-FBS) were obtained from Kalen Biomedical, LLC (Germantown, MD, USA). Sulfo-NHS-LC-biotin and desalting spin column were obtained from ThermoFisher (Waltham, MA, USA). The sources of antibodies were listed in the Supplementary Table 1. Primers were synthesized by IDT (Coralville, IA, USA) and listed in Supplementary Table 2. The animal protocol was approved by the IACUC committee at the University of South Carolina, Columbia. C57BL/6J wild type and LDL receptor (LDLR) KO (B6.129S7-Ldlr^tm1her/J) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). The generation of Spp virus was previously reported [22 (link)].