The intracellular malondialdehyde (MDA) concentration was assessed using a lipid peroxidation colorimetric assay kit purchased from Biovision according to the manufacturer's instructions.
Lipid peroxidation colorimetric assay kit
Manufactured by Abcam
Sourced in United States
The Lipid Peroxidation Colorimetric Assay Kit is a laboratory equipment product designed to measure the level of lipid peroxidation in samples. It provides a quantitative colorimetric assay to determine the concentration of lipid peroxidation byproducts.
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4 protocols using lipid peroxidation colorimetric assay kit
1
Colorimetric Intracellular MDA Assay
2
Serum Lipid Peroxidation Assessment
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The extent of lipid peroxidation in serum was determined using a Lipid Peroxidation
Colorimetric Assay Kit (BioVision, Inc., Milpitas, CA, USA). The degree of lipid
peroxidation was measured indirectly by determining the levels of malondialdehyde in
serum. The assay was carried out according to the manufacturer’s instructions.
Colorimetric Assay Kit (BioVision, Inc., Milpitas, CA, USA). The degree of lipid
peroxidation was measured indirectly by determining the levels of malondialdehyde in
serum. The assay was carried out according to the manufacturer’s instructions.
3
Evaluation of Metabolic Biomarkers in Mice
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A portion of the whole blood collected at the sacrifice of all experimental animals was used for hemoglobin A1c (HbA1c) measurement, using a Mouse Hemoglobin A1c Kit (Crystal Chem Inc., USA). The remaining whole blood was centrifuged (3,000 rpm at 4°C for 20 min) to collect serum samples. Glucose Assay Kits, Insulin (Mouse) ELISA Kits, Protein Carbonyl Content Assay Kit (Biovision), and a TNF-α and IL-1β R&D Duoset ELISA kit (R&D Systems, Minneapolis, MN, USA) were used for serum or urine analysis.
The isolated liver was homogenized and lipid peroxidation was measured using the Lipid Peroxidation Colorimetric Assay Kit (Biovision). All experiments were conducted according to the specifications of the manufacturers.
The isolated liver was homogenized and lipid peroxidation was measured using the Lipid Peroxidation Colorimetric Assay Kit (Biovision). All experiments were conducted according to the specifications of the manufacturers.
4
Spectrophotometric Measurement of MDA
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Malondialdehyde (MDA) was measured using a trade reactive kit (Lipid Peroxidation Colorimetric Assay Kit, Catalogue No. K739-100; BioVision Corporation) based on spectrophotometric measurement at 532 nm of pink color formed by reaction with thiobarbituric acid. The measurement interval was 0.1e20.0 nmol/L [17] .
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